NDUFB8 Antibody - BSA Free

Western Blot: NDUFB8 Antibody [NBP3-05965] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa

Immunocytochemistry/Immunofluorescence: NDUFB8 Antibody [NBP3-05965] - Staining of NDUFB8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NDUFB8 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: NDUFB8 Antibody [NBP3-05965] - Staining of human NDUFB8 in human kidney with rabbit polyclonal antibody at 1:100 dilution.

Immunoprecipitation: NDUFB8 Antibody [NBP3-05965] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-NDUFB8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NDUFB8 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDUFB8 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human NDUFB8 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFB8
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for NDUFB8 Antibody - BSA Free

• CI-ASHImitochondrial
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8 (19kD, ASHI)
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• NADH:ubiquinone oxidoreductase ASHI subunit
• NADH-ubiquinone oxidoreductase ASHI subunit

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFB8