Neurofibromin 1 Antibody - BSA Free

Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).

Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155] - NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Neurofibromin 1 Antibody - BSA Free Summary

• Immunogen: An N-terminal peptide of Neurofibromin 1 (within amino acids 1-50). [Uniprot: P21359]
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NF1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 23910086)
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1:500-1:2000
• Application Notes: In Western blot, a band-set at 220-240 kDa and a higher MW band at 450 kDa (uncharacterized) can be seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
  
  For IHC, antigen retrieval is not required for immunostaining of frozen sections. Antigen retrieval with trypsin or citrate improves immunostaining of paraffin sections.
• Control Peptide:

  Neurofibromin 1 Peptide (NB300-155PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for Neurofibromin 1 Antibody - BSA Free

• DKFZp686J1293
• FLJ21220
• Neurofibromatosis-related protein NF-1
• neurofibromin 1
• neurofibromin
• NFNS
• VRNF
• WSS

Background

Neurofibromin 1 (NF1), the Nf1 gene product, is a large protein which contains a central Ras-GTPase-activating (RasGAP) domain and is suggested to function as a negative regulator of proto-oncogene RAS and downstream effectors. It stimulates the GTPase activity of RAS and acts as regulator of RAS signaling. Consistent with its biochemical activity, NF1 functions as a tumor-suppressor gene in juvenile myelomonocytic leukemia (JMML), and diseased BM cells exhibit aberrant Raf/MEK/ERK signaling. Moreover, NF1 shows greater affinity for Ras GAP, but lower specific activity. Besides JMML, defects in NF1 have also been linked to neurofibromatosis type 1 (NF1), Watson syndrome (WS), familial spinal neurofibromatosis (FSNF), neurofibromatosis-Noonan syndrome (NFNS), colorectal cancer (CRC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurofibromin 1 Antibody (NB300-155)(4)

Publications using NB300-155

• Yoshioka N, Gros E, Li HR et al. Efficient Generation of Human iPSCs by a Synthetic Self-Replicative RNA. Cell Stem Cell 2013-08-01 [PMID: 23910086] (IF/IHC, ICC/IF, Mouse)
• Wallace MD, Pfefferle AD, Shen L et al. Comparative Oncogenomics Implicates the Neurofibromin 1 Gene (NF1) as a Breast Cancer Driver. Genetics 2012-10-01 [PMID: 22851646]
• Kuorilehto, T et al. NF1 gene expression in mouse fracture healing and in experimental rat pseudarthrosis. J Histochem Cytochem;54(3):363-70. 2006-03-01 [PMID: 16314442] (IHC-P, Mouse, Rat)
• Kuorilehto T, Nissinen M, Koivunen J et al. NF1 tumor suppressor protein and mRNA in skeletal tissues of developing and adult normal mouse and NF1-deficient embryos. J Bone Miner Res. 2004-06-01 [PMID: 15125795] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: NF1
• Entrez:
  • 4763(Human)
  • 18015(Mouse)
  • 24592(Rat)
• Uniprot:
  • P21359(Human)