Neurolysin Antibody (OTI1D6)

Western Blot: NLN Antibody (1D6) [NBP2-01693] Analysis of extracts (35ug) from 9 different cell lines by using anti-NLN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: NLN Antibody (1D6) [NBP2-01693] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NLN.

Immunohistochemistry-Paraffin: NLN Antibody (1D6) [NBP2-01693] - Staining of paraffin-embedded Human tonsil using anti-NLN mouse monoclonal antibody.

Flow Cytometry: NLN Antibody (1D6) [NBP2-01693] - Analysis of Jurkat cells, using anti-NLN antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: NLN Antibody (1D6) [NBP2-01693] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN.

Immunohistochemistry-Paraffin: NLN Antibody (1D6) [NBP2-01693] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NLN mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NLN Antibody (1D6) [NBP2-01693] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NLN mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NLN Antibody (1D6) [NBP2-01693] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NLN mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Neurolysin Antibody (OTI1D6) Summary

• Immunogen: Full length human recombinant protein of human NLN(NP_065777) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NLN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 80.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neurolysin Antibody (OTI1D6)

• AGTBP
• AGTBPFLJ23002
• angiotensin binding protein
• Angiotensin-binding protein
• DKFZp564F123
• EC 3.4.24
• EC 3.4.24.16
• KIAA1226EP24.16
• MEP
• mitochondrial
• MOP
• neurolysin (metallopeptidase M3 family)
• Neurolysin
• Neurotensin Endopeptidase
• NLN
• Oligopeptidase M

Background

This gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurolysin Antibody (NBP2-01693)(1)

Publication using NBP2-01693

• Kobayashi R, Tohda C. Extracellular Cytosolic Aspartate Aminotransferase Promotes Axonal Growth and Object Recognition Memory Neurochem. Res. 2017-08-29 [PMID: 28852933] (Human)

Bioinformatics

• Gene Symbol: NLN
• Entrez:
  • 57486(Human)