NFIB Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.

Western Blot: NFIB Antibody [NBP1-81000] - Analysis in human cell line SH-SY5Y.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NFIB Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTP
• Predicted Species: Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFIB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) Reported in scientific literature (PMID: 24801505)
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry Reported in scientif literature (PMID: 26021864)
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: ICC/IF Fixation Permeabilization: PFA/Triton X-100
• Control Peptide:

  NFIB Protein (NBP1-81000PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24553933).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NFIB Antibody - BSA Free

• CCAAT-box-binding transcription factor
• CTF
• HMGIC/NFIB
• NF-I/B
• NFI-B
• NFIB2
• NFIB3
• NFI-RED
• nuclear factor 1 B-type
• Nuclear factor 1/B
• nuclear factor I/BNF1-B
• TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFIB Antibody (NBP1-81000)(8)

Publications using NBP1-81000

• ES Liau, S Jin, YC Chen, WS Liu, M Calon, S Nedelec, Q Nie, JA Chen Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons Oncogene, 2023-01-03;14(1):46. 2023-01-03 [PMID: 36596814] (Immunohistochemistry, Mouse)
• Gokey J, Snowball J, Sridharan A Et al. YAP regulates alveolar epithelial cell differentiation and AGER via NFIB/KLF5/NKX2-1 iScience 2021-09-01 [PMID: 34466790]
• Nagel S, Pommerenke C, Meyer C et al. Establishment of the TALE-code reveals aberrantly activated homeobox gene PBX1 in Hodgkin lymphoma PloS one 2021-02-04 [PMID: 33539429] (WB, Human)
• Hayashi M, Hinckley CA, Driscoll SP, Moore NJ. Graded Arrays of Spinal and Supraspinal V2a Interneuron Subtypes Underlie Forelimb and Hindlimb Motor Control. Neuron. 2018-02-21 [PMID: 29398364] (Immunohistochemistry, Mouse)
• Bunt J, Lim JW, Zhao L et al. PAX6 does not regulate Nfia and Nfib expression during neocortical development. Sci Rep 2015-05-29 [PMID: 26021864] (IF/IHC, Mouse)
• Tsai PC, Bake S, Balaraman S et al. MiR-153 targets the nuclear factor-1 family and protects against teratogenic effects of ethanol exposure in fetal neural stem cells. Biol Open 2014-07-25 [PMID: 25063196] (ICC/IF, Mouse)
• Grabowska MM, Elliott AD, DeGraff DJ et al. NFI Transcription Factors Interact with FOXA1 to Regulate Prostate-Specific Gene Expression. Mol Endocrinol 2014-06-01 [PMID: 24801505] (Chemotaxis, Human)
• Piper M, Barry G, Harvey TJ et al. NFIB-Mediated Repression of the Epigenetic Factor Ezh2 Regulates Cortical Development. J Neurosci 2014-02-19 [PMID: 24553933] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: NFIB
• Entrez:
  • 4781(Human)
• Uniprot:
  • O00712(Human)