NFIB Antibody - BSA Free

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Immunoprecipitation: NFIB Antibody [NBP2-04121] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFIB ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

NFIB Antibody - BSA Free Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 225 and 275 of human Nuclear Factor I/B using the numbering given in entry NP_005587.2 (GeneID 4781).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFIB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-10 mcg/mg
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NFIB Antibody - BSA Free

  • CCAAT-box-binding transcription factor
  • CTF
  • HMGIC/NFIB
  • NF-I/B
  • NFI-B
  • NFIB2
  • NFIB3
  • NFI-RED
  • nuclear factor 1 B-type
  • Nuclear factor 1/B
  • nuclear factor I/BNF1-B
  • TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NFIB
Uniprot