NFX1 Antibody (1D12) - Azide and BSA Free

Western Blot: NFX1 Antibody (1D12) [H00004799-M01] - NFX1 monoclonal antibody (M01), clone 1D12 Analysis of NFX1 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: NFX1 Antibody (1D12) [H00004799-M01] - Analysis of monoclonal antibody to NFX1 on HeLa cell. Antibody concentration 30 ug/ml.

ELISA: NFX1 Antibody (1D12) [H00004799-M01] - Detection limit for recombinant GST tagged NFX1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NFX1 Antibody (1D12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NFX1 (NP_002495, 981 a.a. ~ 1080 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREM
• Localization: Nuclear
• Specificity: NFX1 - nuclear transcription factor, X-box binding 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFX1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NFX1 Antibody (1D12) - Azide and BSA Free

• DKFZp779G2416
• EC 6.3.2.-
• MGC20369
• NFX2transcriptional repressor NF-X1
• Nuclear transcription factor, X box-binding protein 1
• nuclear transcription factor, X-box binding 1

Background

MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFX1
• Entrez:
  • 4799(Human)
• OMIM:
  • 603255(Human)