Nitrotyrosine Antibody

Immunohistochemistry-Frozen: Nitrotyrosine Antibody [NB100-2899] - Rat left ventricle embedded in OCT, flash-frozen in liquid nitrogen and stored at -80C. 8 um sections cut using a cryostat, fixed with ice-cold acetone, blocked using 2% BSA in PBS with 5% normal goat serum, followed by incubation with nitrotyrosine antibody at 1:50 dilution overnight at 4C. Secondary antibody was goat anti-rabbit Alexa Fluor 488 at 1:250 dilution for 1hr at RT. IHC-Fr image submitted by a verified customer review.

• Reactivity: Rt, All-NA
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Nitrotyrosine Antibody Summary

• Immunogen: KLH-nitrotyrosine
• Specificity: The nitrotyrosine antibody reacts with free and bound nitrotyrosine. There is no cross-reactivity with free and bound tyrosine.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1 000 - 1:20 000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:200 - 1:2000
• Application Notes: Nitrotyrosine antibody validated for IHC-Fr from a verified customer review.

Reactivity Notes

Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in distilled water.

Alternate Names for Nitrotyrosine Antibody

• Nitrotyrosine

Background

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Nitrotyrosine Antibody (NB100-2899) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer IHC-Fr Rat 08/19/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult heart
• Species: Rat
• Lot: 15K7

Comments

• Comments: rat left ventricle embedded in OCT, then flash-frozen in liquid nitrogen and stored at -80C. 8 um sections cut using a cryostat. sections fixed with ice-cold acetone, blocked using 2%BSA in PBS with 5% normal goat serum, followed by incubation with 1:50 dilution overnight at 4C. Secondary antibody was goat anti-rabbit Alexa Fluor 488 from ThermoFisher Scientific at 1:250 dilution for 1hr at RT.