Nogo-A Antibody (387521) [Unconjugated]

Nogo‑A was detected in immersion fixed frozen sections of rat embryo (E18) using Mouse Anti-Rat Nogo‑A Monoclonal Antibody (Catalog # MAB3098) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to spinal cord and dorsal root ganglia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Rt
• Applications: IHC
• Clone: 387521
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Nogo-A Antibody (387521) [Unconjugated] Summary

• Immunogen: KLH-coupled rat Nogo-A synthetic peptide
  SYDSIKLEPENPPPYEEA (aa 623-640)
  Accession # Q9JK11
• Specificity: Detects rat Nogo-A in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: Rtn4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nogo-A Antibody (387521) [Unconjugated]

• ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X
• NI220
• NogoA
• Nogo-A
• RTN4
• RTN4A

Background

Nogo-A, also known as Reticulon-4, is the longest of four splice variants of Nogo. It is a CNS myelin-associated neurite outgrowth inhibitor that is highly expressed in oligodendrocytes. Nogo-A is synthesized as an 1163 amino acid protein and lacks a signal peptide. Within conserved C-terminal reticulon homology domain (RHD), two transmembrane domains, which are separated by a 66 amino acid extracellular loop, exist. Both the N-terminal domain and the 66 amino acid domain (Nogo-66) can be detected on the cell surface and show neurite outgrowth inhibitory activity. The amino acid sequence of rat Nogo-A N-terminal domain is 76% identical to that of human Nogo-A. Within the RHD domain, rat Nogo-A shares 99% and 97% amino acid sequence identity with mouse and human Nogo, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nogo-A Antibody (MAB3098)(3)

Publications using MAB3098

• Owen G. James, Bhuvaneish T. Selvaraj, Dario Magnani, Karen Burr, Peter Connick, Samantha K. Barton et al. iPSC-derived myelinoids to study myelin biology of humans Developmental Cell 2021-05-03 [PMID: 33945785]
• Manideep Chavali, Maria José Ulloa-Navas, Pedro Pérez-Borredá, Jose Manuel Garcia-Verdugo, Patrick S. McQuillen, Eric J. Huang et al. Wnt-Dependent Oligodendroglial-Endothelial Interactions Regulate White Matter Vascularization and Attenuate Injury Neuron 2020-12-01 [PMID: 33086038]
• Yi Zhang, Yan Huang, Anna Cantalupo, Paula S. Azevedo, Mauro Siragusa, Jacek Bielawski et al. Endothelial Nogo-B regulates sphingolipid biosynthesis to promote pathological cardiac hypertrophy during chronic pressure overload JCI Insight 2016-04-21 [PMID: 27158676]

Bioinformatics

• Gene Symbol: Rtn4
• Uniprot:
  • Q9JK11()