Nogo-C Antibody

Western blot shows lysates of human skeletal muscle. PVDF Membrane was probed with 1 µg/mL of Human Nogo-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5766) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nogo-C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Nogo-C was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Human Nogo-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5766) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Nogo-C Antibody Summary

• Immunogen: 11 aa unique to the N-terminus of human Nogo-C synthetic peptide
• Specificity: Detects Nogo-C in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Nogo-A and rhNogo-B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: RTN4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nogo-C Antibody

• ASY;Nbla00271;Nbla10545;NI220/250;NOGO;NSP;NSP-CL;Reticulon-4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X
• NogoC
• Nogo-C
• RTN4C

Background

Nogo belongs to the reticulon family of transmembrane proteins, which lack an N-terminal signal sequence and possess a 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif. They show punctate intracellular distribution within the ER that is reminescent of a reticulum. The five Nogo isoforms vary from 199 (Nogo-C) to 1192 (Nogo-A; also called reticulon-4) aa in humans. Nogo-B or -C may form complexes with Nogo-A, and all may be expressed in neurons, endothelial cells, oligodendrocytes, fibroblasts and myoblasts. Of 11 aa unique to the N-terminus of human Nogo-C, 9 and 10 aa are identical in mouse and rat Nogo-C, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nogo-C Antibody (AF5766)(1)

Publication using AF5766

• Z Ahmed, EL Suggate, A Logan, M Berry Retinal Ganglion Cell Survival and Axon Regeneration after Optic Nerve Transection is Driven by Cellular Intravitreal Sciatic Nerve Grafts Cells, 2020-05-27;9(6):. 2020-05-27 [PMID: 32471105] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: RTN4