Nrf2 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 µM MG 132 (Catalog # 1748) for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Nrf2 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Nrf2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Nrf2
  Met17-Asn605
  Accession # Q16236
• Specificity: Detects human Nrf2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse Nrf2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NFE2L2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2-2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nrf2 Antibody [Unconjugated]

• HEBP1
• NFE2L2
• NF-E2-related factor 2
• NFE2-related factor 2
• NRF2 erythroid derived 2, like 2
• Nrf2
• nuclear factor (erythroid-derived 2)-like 2
• nuclear factor erythroid 2-related factor 2
• nuclear factor erythroid-derived 2-like 2

Background

NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nrf2 Antibody (AF3925)(10)

Publications using AF3925

• Zinflou, C;Rochette, PJ; Indenopyrene and Blue-Light Co-Exposure Impairs the Tightly Controlled Activation of Xenobiotic Metabolism in Retinal Pigment Epithelial Cells: A Mechanism for Synergistic Toxicity International journal of molecular sciences 2023-12-12 [PMID: 38139215] (Western Blot, Human)
• Vicente Andreu-Fernández, Mariona Serra-Delgado, Laura Almeida-Toledano, Àgueda García-Meseguer, Melina Vieiros, Anna Ramos-Triguero et al. Effect of Postnatal Epigallocatechin-Gallate Treatment on Cardiac Function in Mice Prenatally Exposed to Alcohol Antioxidants (Basel) 2023-05-09 [PMID: 37237934]
• Gloria Mas, Na Man, Yuichiro Nakata, Concepcion Martinez-Caja, Daniel L Karl, Felipe Beckedorff et al. The SWI/SNF chromatin remodeling subunit DPF2 facilitates NRF2-dependent anti-inflammatory and anti-oxidant gene expression Journal of Clinical Investigation 2023-07-03 [PMID: 37200093] (Chromatin Immunoprecipitation (ChIP), Mouse)
• Liao YH, Wu JT, Hsieh IC et al. ARMS-NF-?B signaling regulates intracellular ROS to induce autophagy-associated cell death upon oxidative stress iScience 2023-02-17 [PMID: 36798436]
• Liao YH, Wu JT, Hsieh IC et al. ARMS-NF-?B signaling regulates intracellular ROS to induce autophagy-associated cell death upon oxidative stress iScience 2023-02-17 [PMID: 36798436]
• Jing Liu, Lulu Zhao, Hongying Cai, Zitao Zhao, Yongbao Wu, Zhiguo Wen et al. Antioxidant and Anti-Inflammatory Properties of Rubber Seed Oil in Lipopolysaccharide-Induced RAW 267.4 Macrophages Nutrients 2022-03-24 [PMID: 35405962]
• Jing Liu, Lulu Zhao, Hongying Cai, Zitao Zhao, Yongbao Wu, Zhiguo Wen et al. Antioxidant and Anti-Inflammatory Properties of Rubber Seed Oil in Lipopolysaccharide-Induced RAW 267.4 Macrophages Nutrients 2022-03-24 [PMID: 35405962] (Western Blot, Mouse)
• David A. Rhodes, Hung-Chang Chen, James C. Williamson, Alfred Hill, Jack Yuan, Sam Smith et al. Regulation of Human gamma δ T Cells by BTN3A1 Protein Stability and ATP-Binding Cassette Transporters Frontiers in Immunology 2018-04-04 [PMID: 29670629]
• Eejung Kim, Nina Ilic, Yashaswi Shrestha, Lihua Zou, Atanas Kamburov, Cong Zhu et al. Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles Cancer Discovery 2016-07-01 [PMID: 27147599]
• Hong Gao, Michael R. Frost, John T. Siegwart, Thomas T. Norton Patterns of mRNA and protein expression during minus-lens compensation and recovery in tree shrew sclera Mol Vis 2011-04-12 [PMID: 21541268]

Bioinformatics

• Gene Symbol: NFE2L2
• Uniprot:
  • Q16236()