Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free

Western Blot: Nurr1 Antibody [NB110-40415] - Western blot detection of Nurr1 in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Nurr1 polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Peptide absorption control lane 3.

Immunohistochemistry: Nurr1 Antibody [NB110-40415] - Nurr1 staining with Nurr1 polyclonal antibody at a 1:100 dilution in adult rat brain hippocampus. Tissue was fixed in paraformaldehyde and cut into 20 micron sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide comprising residues 513-526 [TERHGLKEPKRVEE] of the human Nurr1 protein. Based on 100% sequence homology, this antibody is also expected to cross react with rat and mouse Nurr1.
• Specificity: This is specific to the Nurr1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR4A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:200
  • Immunoprecipitation
  • Western Blot 1:500- 1:2000
• Application Notes: This antibody is useful for Western Blot and Immunohistochemistry. Use in Immunoprecipitation reported in scientific literature (PMID 18784308)

Reactivity Notes

Human and Rat. Other species not yet tested. Mouse reactivity reported in scientific literature (PMID: 18784308).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free

• human homolog of
• NGFI-B beta
• NOT
• NR4A2
• nuclear receptor subfamily 4, group A, member 2
• Nurr1
• orphan nuclear receptor NR4A2
• Orphan nuclear receptor NURR1
• T-cell nuclear receptor NOT
• TINUR
• transcriptionally inducible nuclear receptor related 1
• Transcriptionally-inducible nuclear receptor

Background

The Nurr1 gene encodes a member of the steroid-thyroid hormone- retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson's. Nurr1 is a stem cell marker and cooperates with PITX3 to promote terminal maturation of murine and human embryonic stem cell cultures to a midbrain dopamine neuron phenotype. In addition, Nurr1 is involved in the regulation of corticotropin-releasing hormone (CRH), which may be linked to and associated with rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nurr1/NGFI-B beta/NR4A2 Antibody (NB110-40415)(4)

Publications using NB110-40415

• Finney CA, Wilkins HM, Swerdlow RH et Al. Activators of neurotoxic microglia in neurodegeneration: is the answer in blood? Immunol Cell Biol 2023-09-01 [PMID: 37524376]
• Servaas NH, Hiddingh S, Chouri E et al. Nuclear Receptor Subfamily 4A Signaling as a Key Disease Pathway of CD1c+?Dendritic Cell Dysregulation in Systemic Sclerosis Arthritis & rheumatology (Hoboken, N.J.) 2023-02-01 [PMID: 36482877] (Immunoprecipitation, Mouse)
• Lilley CM, Alarcon A, Ngo MH et al. Orphan Nuclear Receptor NR4A2 Is Constitutively Expressed in Cartilage and Upregulated in Inflamed Synovium From hTNF-Alpha Transgenic Mice Frontiers in Pharmacology 2022-04-20 [PMID: 35529439] (Immunoprecipitation, Mouse)
• Luo Y, Xing F, Guiliano R et al. Identification of a Novel Nurr1-Interacting Protein. J Neurosci;28(37):9277-9286. 2008-01-01 [PMID: 18784308] (IP, Mouse)

Review for Nurr1/NGFI-B beta/NR4A2 Antibody (NB110-40415) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 05/23/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: joints
• Species: Mouse
• Lot: 2930

Comments

• Comments: antigen retrieval
  working concentration: 4ug/mL

Bioinformatics

• Gene Symbol: NR4A2
• Entrez:
  • 4929(Human)
  • 54278(Rat)