Olig1 Antibody - Azide and BSA Free

Western Blot: OLIG1 Antibody [NBP1-28666] - Neonatal rat brain lysate showing specific immunolabeling of the ~ 27k Olig1 protein.

Immunocytochemistry/Immunofluorescence: Olig1 Antibody [NBP1-28666] - Immunostaining of primary rat oligodendrocytes using anti-Olig1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Olig1 Antibody - Azide and BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Recombinant mouse Olig1. Accession # Q9JKN5
• Specificity: Specific for endogenous levels of the ~27 kDa Olig1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OLIG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunoprecipitation 1:50
  • Western Blot 1:3000
• Application Notes: Detects ~27 kDa Olig1 protein in Western blots.
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 200mM Tris (pH 8.0) and 0.1M Glycine
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Olig1 Antibody - Azide and BSA Free

• BHLHB6
• bHLHe21
• Class B basic helix-loop-helix protein 6
• Class E basic helix-loop-helix protein 21
• helix-loop-helix protein, class B, 6
• Olig1
• oligo1
• oligodendrocyte lineage transcription factor 1
• oligodendrocyte transcription factor 1
• oligodendrocyte-specific bHLH transcription factor 1

Background

Olig genes have been identified as the earliest known markers of oligodendrocyte lineage determination to date (Zhou et al., 2000). Olig1 is a transcription factor which promotes formation and maturation of oligodendrocytes, especially within the brain. It is expressed in the ventral spinal cord as early as 9.5 dpc and by 15.5 dpc, olig1 is dispersed throughout the gray matter. In the postnatal brain, it is present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Olig1 has been demonstrated as necessary in the repair of brain lesions in patients with multiple sclerosis (Arnett et al. 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Olig1 Antibody (NBP1-28666)(1)

Publication using NBP1-28666

• Motizuki M, Isogaya K, Miyake K et al. Oligodendrocyte transcription factor 1 (Olig1) is a Smad cofactor involved in cell motility induced by transforming growth factor-beta. J Biol Chem 2013-05-17 [PMID: 23720758] (WB, Mouse)

Bioinformatics

• Gene Symbol: OLIG1
• Entrez:
  • 50914(Mouse)
  • 60394(Rat)
• Uniprot:
  • Q9JKN5(Mouse)