Orexin Antibody

Immunohistochemistry-Paraffin: Orexin Antibody [NBP1-02594] - Staining of human brain, hypothalamus tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Product Discontinued

Orexin Antibody Summary

• Immunogen: Synthetic peptide, PLPDCCRQKTCSC, corresponding to amino acids 2-14 of human Orexin conjugated to KLH.
• Epitope: aa 2-14
• Specificity: Human Orexin, an excitatory neuropeptide linked to alertness and wakefulness in humans.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HCRT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200-1:800
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 9.7 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Orexin Antibody

• hypocretin (orexin) neuropeptide precursor
• NRCLP1
• orexin
• OXHcrt
• PPORX
• PPOXHypocretin

Background

Orexin, or hypocretin (HCRT), is a hypothalamic neuropeptide precusor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, are mediators in the central feedback mechanism that regulates feeding behavior.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HCRT
• Entrez:
  • 3060(Human)
• Uniprot:
  • O43612()