ORP150/HSP12A Antibody - BSA Free

Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibodydiluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Meta-analysis of significant metastatic upregulated proteins in acid exosomes through Prognoscan. Confocal immunofluorescence analysis with the indicated antibodies on primary (apical and mid region), and metastatic melanoma biopsies from the same patient. Ki67 labeling was used as internal control. Red, CFL, GSN, HYOU1; blue, DAPI; green, Ki67. Bar 50 um Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-018-0915-z), licensed under a CC-BY license.

Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Rat tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.

Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded mouse fore brain. ORP150 antibody [C2C3], C-term diluted at 1:500.

Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Meta-analysis of significant metastatic upregulated proteins in acid exosomes through Prognoscan. a Kaplan-Meyer curves depicting the Overall Survival of metastatic melanoma patients with high (red lines) or low (green lines) expression of the genes listed in Table 1 that resulted statistically significant. Mentel-Cox p-values for each plot are showed. b Western blotting analysis of ctr & pH 6.0 exosomes (20 μg). Cell lysate was used as positive control. Expression of CFL, GSN & HSP90 alpha beta is shown. Ponceau red is shown for equal exosome protein amount. c confocal immunofluorescence analysis with the indicated antibodies on primary (apical & mid region), & metastatic melanoma biopsies from the same patient. Ki67 labeling was used as internal control. Red, CFL, GSN, HYOU1; blue, DAPI; green, Ki67. Bar 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30290833), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pl
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ORP150/HSP12A Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ORP150/HSP12A. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum lumen
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HYOU1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 111 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ORP150/HSP12A Antibody - BSA Free

• 150 kDa oxygen-regulated protein
• 170 kDa glucose-regulated protein
• DKFZp686N08236
• FLJ97572
• glucose-regulated protein 170
• Grp170
• GRP-170
• HSP12A
• HYOU1
• hypoxia up-regulated 1
• hypoxia up-regulated protein 1
• ORP150
• ORP-150
• ORP150FLJ94899
• oxygen regulated protein (150kD)

Background

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ORP150/HSP12A Antibody (NBP1-32140)(1)

Publication using NBP1-32140

• Boussadia Z, Lamberti J, et al. Acidic microenvironment plays a key role in human melanoma progression through a sustained exosome mediated transfer of clinically relevant metastatic molecules. J Exp Clin Cancer Res 2018-10-05 [PMID: 30290833] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: HYOU1