Ovalbumin Antibody - BSA Free

Western Blot: Ovalbumin Antibody [NB600-922] - Lane 1: Reduced Ovalbumin. Load: 50 ng. Primary antibody: Ovalbumin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin.

Western Blot: Ovalbumin Antibody [NB600-922] - Western blot detection of Ovalbumin from hen egg white using NB600-922 at 1:10,000. The blot was blocked ON at 4 degrees C in 3% BSA. The secondary antibody used was a DyLight 649 Conjugated Goat anti-rabbit IgG (H&L) diluted 1:20,000 and incubated 1 hr RT in blocking buffer for fluorescent western blotting.

Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Dilution: 1:1,000. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.

Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of Lane 1: Ovalbumin. Load: 50 ng per lane. Dilution: 1:1,000. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.

Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of purified egg white proteins (lysozyme, ovalbumin, ovotransferrin). Image from verified customer review.

Western Blot: Ovalbumin Antibody [NB600-922] - Lane 1: 1 ug Ovalbumin protein reduced. Lane 2: 0.25 ug Ovalbumin protein reduced. Lane 3: 1 ug Ovalbumin protein non-reduced. Lane 4: 0.25 ug Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for ovalbumin. Other band(s): none.

• Reactivity: Ch
• Applications: WB, ELISA, IHC, Single-Cell Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ovalbumin Antibody - BSA Free Summary

• Description: Rabbit Anti-Ovalbumin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Ovalbumin [Hen Egg White] (Uniprot: P01012)
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OVAL
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunohistochemistry
  • Single Cell Western 1:10
  • Western Blot 1:500-1:2000
• Application Notes: SCW is validated using murine sarcoma cells

Reactivity Notes

Cross reactivity against Ovalbumin from other sources is unknown

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Ovalbumin Antibody - BSA Free

• Allergen Gal d 2
• CAP 2
• CAP2
• Cytoplasmic antiproteinase 2
• Egg albumin
• Gal d II
• Hen egg white
• PI8
• Plakalbumin
• Protease inhibitor 8
• Serpin B8
• serpin peptidase inhibitor clade B member 8
• SERPINB14
• SERPINB8

Background

Anti Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ovalbumin Antibody (NB600-922)(10)

Publications using NB600-922

• Amaya L, Grigoryan L, Li Z et al. Circular RNA vaccine induces potent T cell responses Proceedings of the National Academy of Sciences of the United States of America 2023-05-16 [PMID: 37155869] (WB, Human)
• Kim Y, Shim J, Park J et al. Sequential verification of exogenous protein production in OVA gene-targeted chicken bioreactors Poultry Science 2023-01-01 [PMID: 36335737] (IHC-P, WB, Chicken)
• Leary N, Walser S, He Y et al. Melanoma-derived extracellular vesicles mediate lymphatic remodelling and impair tumour immunity in draining lymph nodes Journal of extracellular vesicles 2022-02-01 [PMID: 35188342] (WB, Chicken)
• Jin H, Wu Y, Bi S et al. Higher immune response induced by vaccination in Houhai acupoint relates to the lymphatic drainage of the injection site Res Vet Sci 2020-04-01 [PMID: 32224352] (WB)
• Shimazaki Y, Takahashi A. Antibacterial activity of lysozyme-binding proteins from chicken egg white J. Microbiol. Methods 2018-10-03 [PMID: 30291881] (WB, Chicken)
• Gogesch P, Schulke S, Scheurer S et al. Modular MLV-VLPs co-displaying ovalbumin peptides and GM-CSF effectively induce expansion of CD11b+ APC and antigen-specific T cell responses in vitro Mol. Immunol. 2018-05-28 [PMID: 29852456] (WB)
• Uhlig KM, Schulke S, Scheuplein VA et al. Lentiviral protein transfer vectors are an efficient vaccine-platform inducing strong antigen-specific cytotoxic T cell response J. Virol. 2015-06-17 [PMID: 26085166] (WB, Chicken)
• Li JT, Rank MA, Squillace DL, Kita H. Ovalbumin content of influenza vaccines J Allergy Clin Immunol 2010-06-01 [PMID: 20451987] (ELISA, Virus)
• Kaplan A, Joseph K Kinin formation in C1 inhibitor deficiency. J Allergy Clin Immunol 2010-01-06 [PMID: 20381848] (ELISA, Virus)
• Wang J, Zhang Y, Wei J et al. Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response Immunology 2007-06-01 [PMID: 17371544]
  
  Details:
  This citation used the HRP version of this antibody.

FAQs for Ovalbumin Antibody (NB600-922). (Showing 1 - 4 of 4 FAQs).

1. We would like to know if this antibody can be used for IHC-frozen. (I see an antibody has IHC-P listed as an application, can it also be used in IHC-Fr?)
   • We have only validated this antibody for use in IHC-P however it is likely it will also work in IHC-Fr. You may be interested in testing this as we would make you eligble for our Innovators Reward Program. In exchange for a full review of our product with an image, we will issue you a credit for the original purchase price of the antibody.
2. Hi, We are going to develop an ELISA for detecting ovalbumin in vaccines and we plan to use two Ab from yours: NB600-922 as the coating Ab. We would like to know which of your polyclonal HRP anti ovalbumin would be the best to match with NB600-922, or even another proper pair of Ab for ovalbumin quantification. The target concentration is 5 micrograms/ml.
   • We would probably recommend NBP1-05137 as the antibody to use with this as a pair. The reason being we often find it best to run monoclonal and polyclonal antibodies together as the pair in a sandwich ELISA. That way the monoclonal can be used as the capture and the polyclonal as the detection. The reason I would not use the HRP conjugated polyclonal is I believe they are the same antibody just one is conjugated while the other is not. Therefore they would react to the same sites on the protein and compete for binding.
3. We have a customer who is interested in using NB600-922 with her mouse skin samples that have been infused with ovalbumin. Her samples are formalin-fixed/paraffin-embedded. Do you know what method of antigen retrieval is recommended for this product, or do you have any additional info regarding use in IHC-P?
   • We recommend our standard citrate buffer antigen retrieval method or an enzymatic method with alpha-Chymotrypsin. I do not see any special conditions noted by the lab for staining with this one and the fact this is Rabbit on their mouse samples infused with ovalbumin should work well since there won't be any background with the secondary use.
4. Has NB600-922 been tested for cross reactivity with BSA? I would also like additional information regarding the purification method and if the purity of the antibody has been determined.
   • Our second Western blot image was from testing using BSA as a blocking solution, so it looks like it doesn't react to BSA at all. This antibody was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Concentration was determined by UV absorbance at 280 nm.

Bioinformatics

• Gene Symbol: OVAL
• Uniprot:
  • P01012(Chicken)