P2X3/P2RX3 Antibody

Western Blot: P2X3/P2RX3 Antibody [NB100-1658] - In the protein samples of normal urothelium, UPIIIa, P2X3, P2X5, TRPV1, and TRPV4 are expressed. In low grade carcinoma, there is no expression of uroplakins. P2X3 and TRPV4 are expressed as in normal urothelium, while P2X5 is greatly diminished. TRPV1 expression is decreased in comparison to normal urothelium. In the protein samples of high grade carcinoma with lamina propria invasion (pT1) and those with muscularis propria invasion (pT2), the expression patterns were similar and therefore three examples of each are presented here. The expression of uroplakins is negative and expressions of P2X3 and TRPV1 are substantially decreased compared to normal urothelium. The expressions of P2X5 and TRPV4 are the same as in normal urothelium. Western blots were done in duplicate. Image collected and cropped by CiteAb from the following publication (hindawi.com/journals/bmri/2014/805236/), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: P2X3/P2RX3 Antibody [NB100-1658] - Low grade papillary urothelial carcinoma (pTa). P2X3/P2RX3 immunolabeling (red) is positive in all urothelial cell layers with the strongest immunolabeling in the superficial cells (arrows). TRPV4 positive immunolabeling is seen in the compartments of the lamina propria (arrow). Nuclei are labelled blue with DAPI. L = lumen, LP = lamina propria. Scale bars = 50um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2014/805236/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: P2X3/P2RX3 Antibody [NB100-1658] - Non-petidergic fibers expressing P2X3 receptor for ATP are present in lamina II of the contralateral dorsal horn (arrows). The P2X3-positive fibers are eliminated from the rat dorsal horn ipsilateral to the rhizotomy (arrow heads). Spinal cord segment C6. Scale bar 400 um.P2X3 antibody dilution 1:25,000.

Immunocytochemistry/Immunofluorescence: P2X3/P2RX3 Antibody [NB100-1658] - Staining in rat DRG.

Immunocytochemistry/Immunofluorescence: P2X3/P2RX3 Antibody [NB100-1658] - Staining in Dorsal Horn.

Immunocytochemistry/Immunofluorescence: P2X3/P2RX3 Antibody [NB100-1658] - High grade papillary urothelial carcinomas (pT1 or pT2). Antibody against P2X3/P2RX3 weakly labels (green) urothelial cells. INuclei are labelled blue with DAPI. L = lumen. Scale bars = 50um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2014/805236/) licensed under a CC-BY license.

Product Discontinued

P2X3/P2RX3 Antibody Summary

• Immunogen: Synthetic peptide: VEKQSTDSGAYSIGH, corresponding to amino acids 383-397 of Rat P2X3.
• Localization: Plasma membrane
• Specificity: P2X3
• Isotype: IgG
• Clonality: Polyclonal
• Host: Guinea Pig
• Gene: P2RX3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:100-1:2000
• Control:

  P2X3/P2RX3 Overexpression Lysate

Reactivity Notes

Human reactivity reported in scientific literature (PMID:24868547)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for P2X3/P2RX3 Antibody

• ATP receptor
• MGC129956
• P2RX3
• P2X purinoceptor 3
• P2X receptor, subunit 3
• P2X3
• P2X3purinergic receptor P2X3
• purinergic receptor P2X, ligand-gated ion channel, 3
• Purinergic receptor
• purinoceptor P2X3

Background

P2X3 is a subunit in the family of ion channels activated by extracellular ATP. It was cloned from rat dorsal root ganglia. P2X3 has two putative transmembrane domains with intracellular N- and C-termini. It can form homomeric channels and heteromeric channels with other members of the family. P2X3 is expressed in nociceptive sensory neurons

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P2X3/P2RX3 Antibody (NB100-1658)(5)

Publications using NB100-1658

• Yuan Z, Liu X, Zhang Z et al. Activation of GDNF-ERK-Runx1 signaling contributes to P2X3R gene transcription and bone cancer pain iScience 2022-09-01 [PMID: 36072549] (IHC-P, Rat)
• Lee YL, Lin KL, Wu BN et al. Epigallocatechin-3-gallate alleviates bladder overactivity in a rat model with metabolic syndrome and ovarian hormone deficiency through mitochondria apoptosis pathways. Sci Rep 2018-03-29 [PMID: 29599473]
• Guan NN, Sharma N, Hallen-Grufman K, Jager EWH. The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools. J. Cell. Mol. Med. 2018-02-01 [PMID: 29392898] (Human)
• Helley MP, Abate W, Jackson SK et al. The expression of Toll-like receptor 4, 7 and co-receptors in neurochemical sub-populations of rat trigeminal ganglion sensory neurons. Neuroscience. 2015-12-03 [PMID: 26434622] (WB, ICC/IF, Rat)
• Sterle I, Zupancic D, Romih R. Correlation between urothelial differentiation and sensory proteins P2X3, P2X5, TRPV1, and TRPV4 in normal urothelium and papillary carcinoma of human bladder. Biomed Res Int. 2014-05-28 [PMID: 24868547] (WB, IHC-Fr, Human)

Review for P2X3/P2RX3 Antibody (NB100-1658) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Martin Helley IHC-Fr Rat 08/05/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Rat trigeminal ganglion.
• Species: Rat

Bioinformatics

• Gene Symbol: P2RX3
• Entrez:
  • 5024(Human)
  • 81739(Rat)