P2X3/P2RX3 Antibody

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

P2X3/P2RX3 Antibody Summary

• Immunogen: A synthetic peptide from the cytoplasmic domain of mouse P2X3/P2RX3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: P2RX3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:300-1:2000
  • Immunohistochemistry-Paraffin 1:300-1:2000
  • Western Blot 1:300-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for P2X3/P2RX3 Antibody

• ATP receptor
• MGC129956
• P2RX3
• P2X purinoceptor 3
• P2X receptor, subunit 3
• P2X3
• P2X3purinergic receptor P2X3
• purinergic receptor P2X, ligand-gated ion channel, 3
• Purinergic receptor
• purinoceptor P2X3

Background

P2RX3 is a member of the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and transduces ATP-evoked nociceptor activation. P2RX3 is important for peripheral pain responses, and controls urinary bladder volume reflexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for P2X3/P2RX3 Antibody (NBP2-14838) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: GUANGKUO DONG IHC-Fr Mouse 02/10/2020

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse tongue
• Species: Mouse
• Lot: Ordered on 12/10/2019

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***Tissue: Frozen mouse tongue Postfixed in 4% PFA Cyroprotected in 20% sucrose Cryosectioned at 8 um Protocol: · Rinse sections with PBS 3 x 3 min · Fix in ice-cold acetone – 5 min · Rinse 3X3 PBS · Block with 2% donkey serum in PBS · Primary antibody – overnight at 4 deg C o P2X3 at 1:10, 1:50, 1:100 or negative control/no antibody o Mix= 0.05% Triton + 0.2% carrageenan in PBS · Rinse PBS 3x3 min · Secondary antibody: Incubate 1 hour at RT o Alexa fluor donkey anti-goat 488 1:500 o Mix=0.05% Triton + 0.2% carrageenan in PBS Protect from light · Rinse PBS 3X3 min · Counterstain with DAPI – 5 min at room temp · Rinse PBS 3x3 min

Bioinformatics

• Gene Symbol: P2RX3
• Entrez:
  • 5023(Human)