p97/VCP Antibody (5)

Western Blot: p97/VCP Antibody (5) [NB120-11433] - NKCC1 and KCC2 Expression in DLPFC. Expression of K-Cl cotransporter (KCC2) protein in the DLPFC in control subjects (CTL) and subjects with schizophrenia (SCZ) and in subjects with schizophrenia on and off medication for at least 6 weeks. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123158), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5) [NB120-11433] - Analysis of VCP using VCP Monoclonal antibody (5) shows staining in C6 glioma cells. VCP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: p97/VCP Antibody (5) [NB120-11433] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.

Western Blot: p97/VCP Antibody (5) [NB120-11433] - Analysis of CA46 cell lysate.

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5) [NB120-11433] - Analysis of VCP using VCP Monoclonal antibody (5) shows staining in WiDr colon carcinoma cells. VCP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5) [NB120-11433] - VCP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: p97/VCP Antibody (5) [NB120-11433] - Both normal and cancer biopsies of deparaffinized Human brain tissue tissues.

Product Discontinued

p97/VCP Antibody (5) Summary

• Immunogen: Synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP.
• Localization: Cytoplasmic and Nuclear
• Specificity: This detects VCP protein in human, mouse, and rat samples.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCP
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:400
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:2000
• Application Notes: WB: Detects an approx. 97 kDa protein.
• Control:

  p97/VCP Overexpression Lysate

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 27929047).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for p97/VCP Antibody (5)

• 15S Mg(2+)-ATPase p97 subunit
• IBMPFD
• IBMPFDMGC8560
• MGC131997
• p97
• TER ATPase
• TERA
• transitional endoplasmic reticulum ATPase
• valosin containing protein
• Valosin-containing proteinMGC148092
• VCP
• yeast Cdc48p homolog

Background

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p97/VCP Antibody (NB120-11433)(3)

Publications using NB120-11433

• Brody MJ, Vanhoutte D, Bakshi CV et al. Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis. J Biol Chem. 2019-04-21 [PMID: 31006653] (WB, Rat)
• Tiwari A, Copeland CA, Han B et al. Caveolin-1 is an aggresome-inducing protein Sci Rep. 2016-12-07 [PMID: 27929047] (ICC/IF, Monkey)
• Sullivan CR, Funk AJ, Shan D et al. Decreased chloride channel expression in the dorsolateral prefrontal cortex in schizophrenia PLoS ONE 2015-04-01 [PMID: 25826365] (WB, Rat, Human)

Review for p97/VCP Antibody (NB120-11433) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human and Mouse.

reviewed by: Verified Customer WB Human and Mouse 06/29/2017

Summary

• Application: Western Blot
• Sample Tested: 293FT cell lysate,nih3t3 cell lysate
• Species: Human and Mouse
• Lot: rh237363

Bioinformatics

• Gene Symbol: VCP
• Entrez:
  • 7415(Human)
  • 67459(Mouse)
  • 116643(Rat)