PDGF R beta Antibody (PDGFR-B2)

Product Discontinued

PDGF R beta Antibody (PDGFR-B2) Summary

• Immunogen: PDGF Receptor beta purified from pig uterus.
• Localization: Plasma membrane
• Specificity: The antibody recognizes human and pig PDGF Rb, but not mouse or rat. It can be used for immunoperoxidase and immunofluorescence staining of unfixed or acetone-fixed frozen tissue. The antibody induces clustering and down-regulation of PDGF Rb. The antibody does not cross react with PDGF Ra.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFRB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Western Blot: Use at an assay dependent dilution. Predicted molecular weight: 170-185 kDa. Optimal dilutions/concentrations should be determined by the end user.
• Control:

  PDGF R beta Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Raw ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for PDGF R beta Antibody (PDGFR-B2)

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

Platelet-derived growth factor receptors exhibit tyrosine-protein kinase activity and have been implicated in the control of cell proliferation, survival and migration. PDGF receptors, PDGFR-alpha and PDGFR-beta, have 5 extracellular immunoglobulin-like domains and an intracellular tyrosine kinase domain. Upon binding a PDGF, the receptors form homo-and heterodimers. Dimerization of the receptors results in phosphorylation in the complex. More than 10 different SH2-domain-containing molecules have been shown to bind to different autophosphorylation sites in the PDGF-alpha and beta receptors. PDGF alpha receptors are expressed in oligodendrocyte progenitor cells and PDGF beta receptors are expressed on neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for PDGF R beta Antibody (NB120-10847) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Rossana Franzin IHC-P 05/30/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Lot: NB120-10847

Bioinformatics

• Gene Symbol: PDGFRB
• Entrez:
  • 5159(Human)