Phosphoserine Antibody - BSA Free

Western Blot: Phosphoserine Antibody [NB100-1953] - Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserineprotein using Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953). Primary Antibody: Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1uM okadaic acid).

Immunocytochemistry/Immunofluorescence: Phosphoserine Antibody [NB100-1953] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:50 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.

Western Blot: Phosphoserine Antibody [NB100-1953] - ~30 ug SY5Y or N2A whole cell lysate separated on 8% PAGE, blotted and probed with phosphoserine antibody (1:125 in 3% BSA in TBST). Western blot image submitted by a verified customer review.

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.25 mg/ml

Phosphoserine Antibody - BSA Free Summary

• Immunogen: Phosphoserine conjugated to KLH, and phosvitin mixture
• Specificity: Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:250
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500
• Application Notes: Phosphoserine antibody validated for WB from a verified customer review.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol
• Preservative: 0.01 mg Sodium Azide
• Concentration: 0.25 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Phosphoserine Antibody - BSA Free

• Phosphoserine; Phosphothreonine

Background

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phosphoserine Antibody (NB100-1953)(6)

Publications using NB100-1953

• Caratti G, Desgeorges T, Juban G et al. Macrophagic AMPK alpha 1 orchestrates regenerative inflammation induced by glucocorticoids EMBO reports 2022-12-15 [PMID: 36520372] (IP, Mouse)
• Deng G, Nagai Y, Xiao Y et al. Pim-2 kinase influences Tregulatory cell function and stability by mediating Foxp3 N-terminal phosphorylation J Biol Chem 2015-08-31 [PMID: 25987564] (WB, Human)
  
  Details:
  This citation used the HRP version of this antibody.
• Meena RC, Thakur S, Nath S, Chakrabarti A. Tolerance to thermal and reductive stress in Saccharomyces cerevisiae is amenable to regulation by phosphorylation?dephosphorylation of Ubiquitin conjugating enzyme 1 (Ubc1) S97 and S115. Yeast 2011-10-15 [PMID: 21996927] (WB)
• Deng G, Nagai Y, Xiao Y et al. Pim-2 kinase influences Tregulatory cell function and stability by mediating Foxp3 N-terminal phosphorylation J Biol Chem (WB, Human)
  
  Details:
  This publication used the HRP conjugated form of this antibody (Cat# NB100-1981).
• Rao Y, Qin C, Savas AC, Liu Q et Al. Pyrimidine synthesis enzyme CTP synthetase 1 suppresses antiviral interferon induction by deamidating IRF3 Immunity 2024-12-25 [PMID: 39719712]
• Alavi MV Tau phosphorylation and OPA1 proteolysis are unrelated events: Implications for Alzheimer's Disease Biochim Biophys Acta Mol Cell Res 2021-08-17 [PMID: 34400175] (WB, Human, Mouse)

Review for Phosphoserine Antibody (NB100-1953) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human and Mouse.

reviewed by: Marcel Alavi WB Human and Mouse 02/22/2021

Summary

• Application: Western Blot
• Sample Tested: Mouse Neuro2A whole cell lysate,SHSY5Y whole cell lysate,SH-SY5Y whole cell lysate
• Species: Human and Mouse
• Lot: PP186386