PIM1 Antibody (ST0513)

Western Blot: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of PIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: BT-20 cell lysateLane 2: HepG2 cell lysate Lane 3: SW480 cell lysate

Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: ST0513
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PIM1 Antibody (ST0513) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human PIM1 aa 20-60. (SwissProt: P11309 Human; SwissProt: P06803 Mouse)
• Localization: Cytoplasm, Nucleus, Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PIM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Western Blot 1:500-1:2000
• Application Notes: Use in IHC-Fr reported in scientific literature (PMID:35896115).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PIM1 Antibody (ST0513)

• EC 2.7.11.1
• Oncogene PIM1
• PIM
• pim-1 kinase 44 kDa isoform
• pim-1 oncogene (proviral integration site 1)
• pim-1 oncogene
• PIM1
• proto-oncogene serine/threonine-protein kinase pim-1

Background

The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001 [PubMed 11518967]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIM1 Antibody (NBP2-67528)(7)

Publications using NBP2-67528

• Zhu L, Li H, Peng X et Al. Beneficial mechanisms of dimethyl fumarate in autoimmune uveitis: insights from single-cell RNA sequencing J Neuroinflammation 2024-04-29 [PMID: 38684986]
• Miruna Cristian, Mariana Așchie, Anca-Florentina Mitroi, Mariana Deacu, Mădălina Boșoteanu, Gabriela-Izabela Bălțătescu, Andreea-Georgiana Stoica, Anca-Antonela Nicolau, Manuela Enciu, Ana-Maria Crețu, Andreea-Daniela Caloian, Cristian-Ionuț Orășanu, Ionuț Poinăreanu The impact of MYD88 and PIM1 in mature large B-cell non-Hodgkin lymphomas: Defining element of their evolution and prognosis Medicine 2024-02-09 [PMID: 38335426]
• Li Y, Shi L, Zhao F et al. PIM1 attenuates cisplatin-induced AKI by inhibiting Drp1 activation Cellular signalling 2024-01-01 [PMID: 37967691] (ICC/IF, IHC, Mouse)
• Luo Y, Zhu J, Zhao F et al. PIM1 attenuates renal ischemia-reperfusion injury by inhibiting ASK1-JNK/P38 International immunopharmacology 2022-12-10 [PMID: 36513021] (IHC-P, Mouse)
• Li H, Xie L, Zhu L et al. Multicellular immune dynamics implicate PIM1 as a potential therapeutic target for uveitis Nature communications 2022-10-04 [PMID: 36195600] (FLOW, IF/IHC, Mouse)
• Li Z, Wang M, Tan J et al. Single-cell RNA sequencing depicts the local cell landscape in thyroid-associated ophthalmopathy Cell reports. Medicine 2022-07-19 [PMID: 35896115] (IHC-Fr, Human)
• Szydlowski M, Debek S, Prochorec-Sobieszek M et al. PIM Kinases Promote Survival and Immune Escape in Primary Mediastinal Large B-Cell Lymphoma through Modulation of JAK-STAT and NF-kappaB Activity The American journal of pathology 2020-12-08 [PMID: 33307035]

Bioinformatics

• Gene Symbol: PIM1