Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining in human stomach and liver tissues using NBP2-39021 antibody. Corresponding GALNT7 ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human kidney, liver, prostate and stomach using Anti-GALNT7 antibody NBP2-39021 ...read more
Western Blot: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human cell line HaCaT shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human kidney shows very weak positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human prostate shows strong positivity in glandular cells of Golgi apparatus.
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human stomach shows strong positivity in Golgi apparatus in glandular cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GDSQKDIMQRQYLTFKPQTFTYHDPVLRPGILGNFEPKEPEPPGVVGGPGEKAKPLVLGPEFKRAI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GALNT7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Polypeptide GalNac Transferase 7/GALNT7 Protein (NBP2-39021PEP)
Publications
Read Publication using
NBP2-39021 in the following applications:

  • WB
    1 publication

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35087510). Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free

  • EC 2.4.1
  • EC 2.4.1.-
  • EC 2.4.1.37
  • EC 2.4.1.41
  • GalNAcT7
  • GALNAC-T7
  • GALNT7
  • N-acetylgalactosaminyltransferase 7
  • Polypeptide GalNAc transferase 7
  • polypeptide N-acetylgalactosaminyltransferase 7
  • pp-GaNTase 7
  • Protein-UDP acetylgalactosaminyltransferase 7
  • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7
  • UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 7 (GalNAc-T7)

Background

GALNT7 encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Polypeptide GalNac Transferase 7/GALNT7 Antibody (NBP2-39021)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GALNT7
Uniprot