PPAN Antibody - BSA Free

Western Blot: PPAN Antibody [NBP2-93075] - Analysis of extracts of various cell lines, using PPAN . Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: PPAN Antibody [NBP2-93075] - Analysis of A549 cells using PPAN .

Immunohistochemistry-Paraffin: PPAN Antibody [NBP2-93075] - Paraffin-embedded human stomach using PPAN .

Immunohistochemistry-Paraffin: PPAN Antibody [NBP2-93075] - Paraffin-embedded human prostate using PPAN .

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PPAN Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PPAN (NP_064615.3). MGQSGRSRHQKRARAQAQLRNLEAYAANPHSFVFTRGCTGRNIRQLSLDVRRVMEPLTASRLQVRKKNSLKDCVAVAGPLGVTHFLILSKTETNVYFKLMRLPGGPTLTFQVKKYSLVRDVVSSLRRHRMHEQQFAHPPLLVLNSFGPHGMHVKLMATMFQNLFPSINVHKVNLNTIKRCLLIDYNPDSQELDFRHYSIKVVPVGASRGMKKLLQEKFPNMSRLQDISELLATGAGLSESEAEPDGDHNITELPQAVAGRGNMRAQQSAVRLTEIGPRMTLQLIKVQEGVGEGKVMFHSF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPAN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PPAN Antibody - BSA Free

• Brix domain-containing protein 3
• homolog of S. cerevisiae SSF1
• MGC14226
• MGC45852
• peter pan (Drosophila) homolog
• peter pan homolog (Drosophila)
• Peter Pan homolog
• second-step splicing factor 1
• SSF
• Ssf-1
• SSF1BXDC3SSF-1
• SSF2
• suppressor of sterile four 1
• suppressor of SWI4 1 homolog

Background

PPAN is encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. This gene was found to cotranscript with P2RY11/P2Y(11), an immediate downstream gene on the chromosome that encodes a ATP receptor. The chimeric transcripts of this gene and P2RY11 were found to be ubiquitously present and regulated during granulocytic differentiation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPAN