PRDM16/MEL1 Antibody - BSA Free

Western Blot: PRDM16/MEL1 Antibody [NBP1-77096] - Analysis of PRDM16 in K562 cell lysate with PRDM16 antibody at 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: PRDM16/MEL1 Antibody - BSA Free [NBP1-77096] - Immunofluorescence of PRDM16/MEL1 in Human Brain cells with PRDM16/MEL1 antibody at 20 u/mL.

Immunohistochemistry: PRDM16/MEL1 Antibody - BSA Free [NBP1-77096] - Immunohistochemistry of PRDM16/MEL1 in human brain tissue with PRDM16/MEL1 antibody at 2 u/ml.

Immunocytochemistry/ Immunofluorescence: PRDM16/MEL1 Antibody - BSA Free [NBP1-77096] - Immunofluorescence of PRDM16/MEL1 in mouse brain tissue with PRDM16/MEL1 antibody at 20 u/ml.Green: PRDM16/MEL1 Antibody Blue: DAPI staining

Immunohistochemistry: PRDM16/MEL1 Antibody - BSA Free [NBP1-77096] - Immunohistochemistry of PRDM16/MEL1 in mouse brain tissue with PRDM16/MEL1 antibody at 2 u/ml.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PRDM16/MEL1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PRDM16. The immunogen is located within amino acids 1120 - 1170 of PRDM16. Amino Acid Squence: AGKSQDDTVSPAPEPQA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRDM16
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  PRDM16/MEL1 Peptide (NBP1-77096PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PRDM16/MEL1 Antibody - BSA Free

• MDS1/EVI1-like gene 1
• MEL1
• MEL1KIAA1675PFM13MGC166915
• PFM13
• PR domain containing 16
• PR domain zinc finger protein 16
• PR domain-containing protein 16
• PRDM16
• Transcription factor MEL1

Background

PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRDM16/MEL1 Antibody (NBP1-77096)(8)

Publications using NBP1-77096

• Cheng L, Shi L, He C et al. Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1? signaling pathway Chinese journal of natural medicines 2023-11-01 [PMID: 38035937]
• Baskaran P, Nazminia K, Frantz J et al. Mice lacking endogenous TRPV1 express reduced levels of thermogenic proteins and are susceptible to diet-induced obesity and metabolic dysfunction FEBS letters 2021-05-11 [PMID: 33977527] (Western Blot, Mouse)
• Beppu LY, Mooli R, Qu X et al. Tregs facilitate obesity and insulin resistance via a Blimp-1-IL-10 axis JCI insight 2020-12-22 [PMID: 33351782] (WB, Mouse)
• Meyers K, Lopez M, Ho J et al. Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice Sci Rep 2020-09-03 [PMID: 32883997]
• Baskaran P, Covington K, Bennis J et al. Binding Efficacy and Thermogenic Efficiency of Pungent and Nonpungent Analogs of Capsaicin. Molecules 2018-12-04 [PMID: 30518154] (WB, Mouse)
• Baskaran P, Krishnan V, Ren J, Thyagarajan B. Capsaicin Induces Browning of White Adipose Tissue and Counters Obesity by activating TRPV1 dependent mechanism. Br. J. Pharmacol. 2016-05-12 [PMID: 27174467] (WB, Mouse)
• Beppu L The role of Blimp-1 transcriptional regulator in adipose-resident Tregs Thesis 2021-01-01
• Cheng L, Shi L, He C et al. Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels Phytotherapy research : PTR 2022-04-21 [PMID: 35445769]

Bioinformatics

• Gene Symbol: PRDM16
• Entrez:
  • 63976(Human)
• Uniprot:
  • Q9HAZ2(Human)