Proteasome 20S alpha 5 Antibody

Western Blot: Proteasome 20S alpha 5 Antibody [NBP2-97136] - Anti-Proteasome 20S alpha 5 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa

Immunohistochemistry-Paraffin: Proteasome 20S alpha 5 Antibody [NBP2-97136] - Immunochemical staining of human Proteasome 20S alpha 5 in human brain with rabbit polyclonal antibody at 1:15000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: Proteasome 20S alpha 5 Antibody [NBP2-97136] - Proteasome 20S alpha 5 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate Lane B: 0.5 mg MCF7 Whole Cell Lysate 1 ul anti-Proteasome 20S alpha 5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Proteasome 20S alpha 5 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 28 kDa

Product Discontinued

Proteasome 20S alpha 5 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Proteasome 20S alpha 5 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMA5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Proteasome 20S alpha 5 Antibody

• EC 3.4.25.1
• FLJ42315
• macropain subunit zeta
• Macropain zeta chain
• MGC117302
• MGC125802
• MGC125803
• MGC125804
• Multicatalytic endopeptidase complex zeta chain
• proteasome (prosome, macropain) subunit, alpha type, 5
• Proteasome 20S alpha 5
• proteasome alpha 5 subunit
• proteasome component 5
• proteasome subunit alpha type-5
• proteasome subunit zeta
• Proteasome zeta chain
• PSC5
• PSMA5
• ZETA

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMA5