PUMA Antibody - BSA Free

Western Blot: PUMA Antibody [NB100-56370] - Analysis for Puma a (CT) using NB100-56370 at 1:500 against 30 ug/lane of A) Jurkat and B) NIH 3T3 whole cell lysate.

Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells.

Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells. The representative section is showing alveolar area only.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PUMA Antibody - BSA Free Summary

• Immunogen: A portion of amino acids 186-199 (PLPRGHRAPEMEPN) of human PUMA a was used as the immunogen.
• Specificity: The amino acid sequence used as immunogen is 100% homologous in human (PUMA a and b). Human PUMA a is a 193 amino acid protein with a predicted molecular weight of approximately 22 kD. Human PUMA b is a 131 amino acid protein with a predicted molecular weight of approximately 15 kD.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BBC3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:250
  • Immunohistochemistry-Paraffin reported in scientific literature (Karst et al (2005))
  • Western Blot 1:500-1:1000
• Control:

  Jurkat Whole Cell Lysate
  NIH 3T3 Whole Cell Lysate

Reactivity Notes

The amino acid sequence used as immunogen is 78% homologous in rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PUMA Antibody - BSA Free

• BCL2 binding component 3
• bcl-2-binding component 3
• JFY1
• JFY-1
• p53 up-regulated modulator of apoptosis
• PUMAFLJ42994

Background

A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PUMA Antibody (NB100-56370)(6)

Publications using NB100-56370

• Ran N, Gao X, Dong X et al. Effects of exosome-mediated delivery of myostatin propeptide on functional recovery of mdx mice Biomaterials 2020-01-01 [PMID: 32028167] (IF/IHC, Mouse)
• Liao Y, Chu HP, Hu Z et al. Paradoxical roles of elongation factor-2 kinase in stem cell survival. J. Biol. Chem. 2016-07-27 [PMID: 27466362] (WB, Mouse)
• Kim DH, Jung YJ, Lee JE et al. SIRT1 activation by resveratrol ameliorates cisplatin-induced renal injury through deacetylation of p53. Am J Physiol Renal Physiol;301(2):F427-35. 2011-08-01 [PMID: 21593185]
• Speidel D, Helmbold H, Deppert W. Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress. Oncogene. 2006-02-09 [PMID: 16247471] (WB)
  
  Details:
  WB: Fig 3b (NIH3T3).
• Karst AM, Dai DL, Martinka M, Li G. PUMA expression is significantly reduced in human cutaneous melanomas. Oncogene. 2005-02-03 [PMID: 15690057] (IHC-P)
  
  Details:
  Puma (IMG-458). IHC (paraffin): Human dysplastic nevi, primary melanoma, and metastatic melanoma tumor tissues, Fig 1.
• Hershko T, Ginsberg D. Up-regulation of Bcl-2 homology 3 (BH3)-only proteins by E2F1 mediates apoptosis. J Biol Chem. 2004-03-05 [PMID: 14684737] (WB, Mouse)
  
  Details:
  WB: Fig 1C (NIH3T3) & Fig 9 (mouse embryo fibroblasts).

Bioinformatics

• Gene Symbol: BBC3
• Entrez:
  • 27113(Human)
  • 170770(Mouse)