RBBP4/RbAp48 Antibody (JU37-47)

Western Blot: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.Positive control:Lane 1: NIH-3T3 Lane 2: HelaLane 3: Rat brain Lane 4: Mouse testis

Western Blot: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.Positive control:Lane 1: NIH-3T3 Lane 2: HelaLane 3: Rat brain Lane 4: Mouse testis

Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.

Flow Cytometry: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: JU37-47
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RBBP4/RbAp48 Antibody (JU37-47) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human RBBP4/RbAp48 aa 1-98 / 425. (SwissProt: Q09028 Human; SwissProt: Q60972 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RBBP4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RBBP4/RbAp48 Antibody (JU37-47)

• CAF-1 p48
• CAF-1 subunit C
• CAF-1C
• CAF-I p48
• Chromatin assembly factor 1 subunit C
• Chromatin assembly factor I p48 subunit
• chromatin assembly factor/CAF-1 p48 subunit
• histone-binding protein RBBP4
• MSI1 protein homolog
• Nucleosome-remodeling factor subunit RBAP48
• NURF55
• RBAP48
• RBBP4
• RBBP-4
• retinoblastoma binding protein 4
• Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
• Retinoblastoma-binding protein p48

Background

RbAp48 encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBBP4