RBFOX3/NeuN Antibody - BSA Free

Western Blot: RBFOX3/NeuN Antibody [NBP3-05554] - Western blot analysis of whole brain lysates using RBFOX3/NeuN Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain. Bands at 46k and 48 kDa correspond to protein isotypes of RBFOX3/NeuN.

Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP3-05554] - Immunofluorescent analysis of a section of adult rat hippocampus stained with RBFOX3/NeuN Antibody at a dilution of 1:2,000 in red, costained with mouse monoclonal antibdy to MAP2 at a dilution of 1:2,000, in green. Nuclear DNA was revealed in blue using the DAPI stain. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The RBFOX3/NeuN antibody stains the nuclei of neurons in the hippocampus while the MAP2 antibody stains the dendritic processes of neurons.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Eq
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RBFOX3/NeuN Antibody - BSA Free Summary

• Immunogen: N-terminal 100 amino acids of human RBFOX3/NeuN expressed in and purified from E. coli
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RBFOX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry 1:1000-1:5000
  • Western Blot 1:1000-1:2000
• Theoretical MW: 33.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBFOX3/NeuN Antibody - BSA Free

• FLJ56884
• FLJ58356
• Fox-1 homolog C
• FOX3
• FOX-3
• hexaribonucleotide binding protein 3
• HRNBP3
• neuN antigen
• NEUN
• neuronal nuclei antigen
• neuronal nuclei
• RBFOX3
• RNA binding protein fox-1 homolog 3
• RNA binding protein, fox-1 homolog (C. elegans) 3
• RNA binding protein, fox-1 homolog 3

Background

Rbfox3 (also known as Fox-3, Fox1 homolog C, Hrnbp3, and D11Bwg0517e) is one of three mammalian members of the RNA-binding protein Rbfox gene family, all of which are involved in regulating alternative RNA splicing. Originally identified as an epitope, NeuN is located within the N-terminal region (6-15 amino acids) of Rbfox3. Rbfox family members are highly conserved, having a single RNA recognition motif (RRM)-type RNA binding domain (RBD) near the center of the protein sequence. The RBD amino acid (aa) sequence is identical between members, Rbfox1 and Rbfox2, with 4 differences within the 77 aa domain of Rbfox3. The fox3 gene is comprised of 15 exons in humans, 11 exons in rat, with 3 variants in mouse containing 14 exons and another 3 variants containing 15 exons. The Rbfox3 protein is highly conserved across human, mouse, and rat with 98.9% sequence identity between mouse (isoform I) and rat and 83.9% sequence identity between mouse (isoform I) and human. The expression of Rbfox3/NeuN is restricted to the nervous system and has widely been used in stroke research. It is recognized as a marker of mature neuronal cell types in the spinal cord, cerebral cortex, hippocampus, dorsal thalamus, caudate/putamen, and cerebellum. NeuN has been shown to bind DNA and is predominantly nuclear. Differences in immunoreactivity have been reported between Rbfox3/NeuN subtypes in which the 46kDa is mainly found in the nucleus whereas the 48kDa form is primarily distributed in the cytoplasm (1,2).

Rbfox proteins participate in regulation of alternative splicing between family members as well as in autoregulation. While the breadth of brain and muscle specific targets for alternative splicing is well established for Rbfox proteins, those specific to Rbfox3/NeuN along with its alternative splicing mechanism are less understood. Rbfox3 has been shown to regulate neuronal differentiation by alternative splicing of Numb pre-mRNA and has a role in adult neurogenesis. Dysfunctional Rbfox3/NeuN has been associated with various neurological disorders such as neurodevelopmental delay, autism spectrum disorder, Benign rolandic epilepsy (BRE), and cognitive impairments (1,2).

References

1. Duan W, Zhang YP, Hou Z, Huang C, Zhu H, Zhang CQ, Yin Q. (2016) Novel Insights into NeuN: from Neuronal Marker to Splicing Regulator. Mol Neurobiol. 53(3):1637-1647. PMID: 25680637.

2. Su CH, D D, Tarn WY. (2018) Alternative Splicing in Neurogenesis and Brain Development. Front Mol Biosci. 5:12. PMID: 29484299

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBFOX3/NeuN Antibody (NBP3-05554)(8)

Publications using NBP3-05554

• Song-I Seol, Dashdulam Davaanyam, Sang-A Oh, Eun-Hwa Lee, Pyung-Lim Han, Seung-Woo Kim, Ja-Kyeong Lee, Ines Moreno-Gonzalez, Rosanna Dono, Joana A. Loureiro, Claudia Duran-Aniotz Age-Dependent and A beta -Induced Dynamic Changes in the Subcellular Localization of HMGB1 in Neurons and Microglia in the Brains of an Animal Model of Alzheimer’s Disease Cells 2024-01-18 [PMID: 38247880]
• Yadong Wang, Zhaohui Dang, Xu Wang, Yuanyuan Chen, Peng Dong, Gang Liu, Weibin Tan, Zhong Gui, Fan Bu, Feng Lin, Chaozhao Liang Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice Biochemistry and Biophysics Reports 2023-10-31 [PMID: 37965064]
• Bu F, Li Y, Lan S et al. Blocking Pannexin-1 Channels Alleviates Thalamic Hemorrhage-Induced Pain and Inflammatory Depolarization of Microglia in Mice ACS chemical neuroscience 2023-06-28 [PMID: 37377340]
• Enders J, Jack J, Thomas S et al. Ketolysis is required for the proper development and function of the somatosensory nervous system Experimental neurology 2023-04-24 [PMID: 37100111] (IHC, Mouse)
• Jiang W, He F, Ding G, Wu J Elamipretide reduces pyroptosis and improves functional recovery after spinal cord injury CNS neuroscience & therapeutics 2023-04-20 [PMID: 37081763] (ICC/IF)
• Wang Y, Dang Z, Wang X et al. Obacunone Alleviates Chronic Pelvic Pain and Pro-Inflammatory Depolarization of Macrophage Induced by Experimental Autoimmune Prostatitis in Mice Available at SSRN 2023-01-01 (Immunohistochemistry, Mouse)
• Jiang W, He F, Ding G, Wu J Dopamine inhibits pyroptosis and attenuates secondary damage after spinal cord injury in female mice Neuroscience letters Oct 25 2022 [PMID: 36307053] (ICC/IF, Mouse)
• Jiang W, He F, Ding G, Wu J Topotecan Reduces Neuron Death after Spinal Cord Injury by Suppressing Caspase-1-Dependent Pyroptosis Molecular neurobiology Jul 18 2022 [PMID: 35851945]

Bioinformatics

• Gene Symbol: RBFOX3