Recombinant Human IL-12 (linked heterodimer) Protein, CF

Recombinant Human IL-12 (Catalog # 10018-IL) stimulatesproliferation in PHA-activated human T lymphoblasts. The ED50 for this effectis 0.01-0.05 ng/mL.

2 μg/lane of Recombinant Human IL‑12 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 63-75 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-12 (linked heterodimer) Protein, CF Summary

• Additional Information: GS Linker, Defined heterodimer stoichiometry.
• Details of Functionality: Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
  The ED₅₀ for this effect is 0.01-0.05 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human IL-12 protein
  

  Human IL-12p40 (Ile23-Ser328) Accession # P29460.1	GSGSSRGGSGSGGSGGGGSK	Human IL-12p35 (Arg23-Ser219) Accession # P29459.2
  N-terminus		C-terminus

• Accession #: P29460.1 (IL-12p40), P29459.2 (IL-12p35)
• N-terminal Sequence: Ile23
• Structure / Form: GS-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 63-75 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100-200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-12 (linked heterodimer) Protein, CF

• CLMF p35
• CLMF1
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL-12 subunit p35
• IL12
• IL-12
• IL-12, subunit p35
• IL12A
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines (1). The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF (2, 3). These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells (4). These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN‑gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells (5). Current evidence indicates that IL‑12, produced by macrophages in response to infectious agents, is a central mediator of the cell‑mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

1. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.
2. Gearing, D. et al. (1991) Cell 66:9.
3. Merberg, D. et al. (1992) Immunology Today 13:78.
4. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074.
5. Airoldi, I. et al. (2000) Journal of Immunology 165:6880.

Publications for IL-12 (10018-IL)(3)

Publications using 10018-IL

• Arias, AA;Neehus, AL;Ogishi, M;Meynier, V;Krebs, A;Lazarov, T;Lee, AM;Arango-Franco, CA;Yang, R;Orrego, J;Corcini Berndt, M;Rojas, J;Li, H;Rinchai, D;Erazo-Borrás, L;Han, JE;Pillay, B;Ponsin, K;Chaldebas, M;Philippot, Q;Bohlen, J;Rosain, J;Le Voyer, T;Janotte, T;Amarajeeva, K;Soudée, C;Brollo, M;Wiegmann, K;Marquant, Q;Seeleuthner, Y;Lee, D;Lainé, C;Kloos, D;Bailey, R;Bastard, P;Keating, N;Rapaport, F;Khan, T;Moncada-Vélez, M;Carmona, MC;Obando, C;Alvarez, J;Cataño, JC;Martínez-Rosado, LL;Sanchez, JP;Tejada-Giraldo, M;L'Honneur, AS;Agudelo, ML;Perez-Zapata, LJ;Arboleda, DM;Alzate, JF;Cabarcas, F;Zuluaga, A;Pelham, SJ;Ensser, A;Schmidt, M;Velásquez-Lopera, MM;Jouanguy, E;Puel, A;Krönke, M;Ghirardello, S;Borghesi, A;Pahari, S;Boisson, B;Pittaluga, S;Ma, CS;Emile, JF;Notarangelo, LD;Tangye, SG;Marr, N;Lachmann, N;Salvator, H;Schlesinger, LS;Zhang, P;Glickman, MS;Nathan, CF;Geissmann, F;Abel, L;Franco, JL;Bustamante, J;Casanova, JL;Boisson-Dupuis, S; Tuberculosis in otherwise healthy adults with inherited TNF deficiency Nature 2024-08-28 [PMID: 39198650] (Bioassay, Human)
• Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells. iScience, 2021-11-14;24(12):103433. 2021-11-14 [PMID: 34917892] (Bioassay, Human)
• Gao Y, Sun W, Cha X, Wang H &#039;Psoriasis 1&#039; reduces T‑lymphocyte‑mediated inflammation in patients with psoriasis by inhibiting vitamin D receptor‑mediated STAT4 inactivation. Int J Mol Med, 0;46(4):1538-1550. 0 [PMID: 32945358] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • P29460.1 (IL-12p40), P29459.2 (IL-12p35)()