Recombinant Human Integrin alpha V beta 1 Protein, CF

When Recombinant Human Fibronectin (Catalog # 4305-FNB) is coated at2 μg/mL Recombinant Human Integrin alpha V beta 1 (Catalog # 6579-AVB) binds with an ED50 of0.08-0.48 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Integrin alpha V beta 1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Fibronectin (Catalog # 4305-FNB) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Integrin alpha V beta 1 binds with an ED₅₀ of 0.08-0.48 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 1 protein
  

  Human Integrin alpha V (Phe31-Val992) Accession # NP_002201	GGGSGGGSGGGS	Acidic Tail	HHHHHH
  Human Integrin beta 1 (Gln21-Asp728) Accession # P05556	GGGSGGGSGGGS	Basic Tail
  N-terminus			C-terminus

• N-terminal Sequence: Phe31 ( alpha V subunit) & Gln21 ( beta 1 subunit, inferred from enzymatic pyroglutamate treatment revealing Thr22)
• Structure / Form: Noncovalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 115 kDa ( alpha V subunit) & 86 kDa ( beta 1 subunit).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-170 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha V beta 1 Protein, CF

• CD51
• Integrin alpha V beta 1
• integrin subunit alpha V
• MSK8
• VNRA
• VTNR

Background

Integrin alpha V beta 1 is one of five alpha V and twelve beta 1 containing Integrin family adhesion receptor heterodimers (1‑3). The non-covalent heterodimer of 170 kDa alpha V and 130 kDa beta 1/CD29 is present on cells that express both subunits, and dimer formation is dependant on the availability of the individual subunits (4). Since the alpha V and beta 1 subunits are widely expressed, the alpha V beta 1 heterodimer potentially forms in many cell types. The 962 aa human alpha V extracellular domain (ECD) shares 92‑95% aa sequence identity with mouse, rat and bovine alpha V, while the 708 aa human beta 1 ECD shares 92‑96% aa sequence identity with mouse, rat, bovine and feline  beta 1. The alpha V ECD contains an N‑terminal beta ‑propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1). alpha V integrins bind ligands that contain an RGD motif, including vitronectin, fibronectin and osteopontin (4‑9). The relatively weak binding affinity of alpha V beta 1 to vitronectin and fibronectin is thought to facilitate its activity in cyclic binding and release during cell migration (4, 5). In oligodendrocytes, astrocytes and pancreatic beta cells, alpha V beta 1 is expressed early in differentiation when cells are migrating and is down‑regulated when differentiation is complete (5‑7). alpha V beta 1 has also been found to be a receptor for angiopoietin-2 in Tie2-deficient glioma cells, and to mediate cell entry of viruses such as foot‑and‑mouth disease virus and human metaneumovirus (10‑12).

1. Hynes, R. O. (2002) Cell 110:673.
2. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
3. Argraves, W.S. et al. (1987) J. Cell Biol. 1025:1183.
4. Koistinen, P. and J. Heino (2002) J. Biol. Chem. 277:24835.
5. Kaido, T. et al. (2004) J. Biol. Chem. 279:17731.
6. Milner, R. et al. (1996) J. Neurosci. 16:7240.
7. Milner, R. et al. (2001) Mol. Cell. Neurosci. 18:108.
8. Koivisto, L. et al. (2000) Exp. Cell Res. 255:10.
9. Hu, D.D. et al. (1995) J. Biol. Chem. 270:26232.
10. Hu, B. et al. (2006) Cancer Res. 66:775.
11. Jackson, T. et al. (2002) J. Virol. 76:935.
12. Cseke, G. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1566.

Publications for Integrin alpha V beta 1 (6579-AVB)(2)

Publications using 6579-AVB

• Stampolidis P, Ullrich A, Iacobelli S LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene, 2013-12-23;34(1):39-52. 2013-12-23 [PMID: 24362527] (Bioassay, Human)
• Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice. PLoS ONE, 2013-11-18;8(11):e81165. 2013-11-18 [PMID: 24260554] (Binding Assay)