Recombinant Mouse Galectin-3BP/MAC-2BP Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Galectin-3BP/MAC-2BP Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When rmMAC-2BP is coated at 1 μg/mL (100 μL/well), Recombinant Human Galectin‑3 (Catalog # 1154-GA) binds with an apparent K_D <5 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse Galectin-3BP/MAC-2BP protein
  Thr19-Val577, with a C-terminal 6-His tag
• Accession #: NP_035280
• N-terminal Sequence: Thr19
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 63.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and Imidazole with trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Galectin-3BP/MAC-2BP Protein, CF

• 90K
• Basement membrane autoantigen p105
• BTBD17B
• galectin-3-binding protein
• Galectin3BP
• Galectin-3BP
• L3 antigen
• Lectin galactoside-binding soluble 3-binding protein
• lectin, galactoside-binding, soluble, 3 binding protein
• LGALS3BP
• M2BP
• Mac-2 BP
• Mac-2-binding protein
• MAC2BP
• MAC-2BP
• MAC-2-BP
• serum protein 90K
• Tumor-associated antigen 90K

Background

Galectin-3 binding protein (Galectin-3BP), called CyCAP (cyclophilin C-associated protein) in the mouse, is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Mouse Galectin-3BP is generally considered the paralog of human Galectin-3BP, which is also called MAC-2 binding protein (MAC-2BP), or 90 kDa tumor associated antigen (TAA90K or 90K) (1, 2). However, mouse Galectin-3BP binds cyclophilin C but not Galectin-3 (formerly MAC-2), while the opposite is true of human Galectin-3BP (3). The 577 amino acid (aa), 77 - 95 kDa mouse Galectin-3BP contains an 18 aa signal sequence and four definitive domains (1 - 6). Domain 1 is a group A scavenger receptor domain, domain 2 is a BTB/POZ domain that mediates dimerization, and domain 3 is an IVR domain that is also found following the POZ domain in Drosophila kelch protein (4, 5). Little is known about domain 4 (5, 6). Mature mouse Galectin-3BP shares 88%, 69%, 67% and 65% aa identity with rat, human, dog and cow Galectin-3BP, respectively. The Galectin-3BP dimer interface residues are generally conserved between mouse and human, so linear dimers that occur in humans are also likely in the mouse (5 - 7). Human Galectin-3BP also forms ring-shaped decamers and dodecamers. Mouse Galectin-3BP is widely expressed and modifies innate immune reactions. It is stimulated in macrophages by adhesion, TNF-alpha or IFN-gamma (2). Mice deficient in Galectin-3BP secrete increased amounts of IL-12, TNF-alpha and IFN-gamma and have lower survival in response to endotoxin (8). Galectin-3BP is induced in rat skin fibroblasts, keratinocytes, macrophages and lymphocytes during wound healing, and wounded Galectin-3BP-deficient mice are slow to heal (9). Rat Galectin-3BP is upregulated after cerebral ischemia (10). Ligands for human Galectin-3BP include some other galectins, collagens and integrins, but remain to be tested for mouse Galectin-3BP binding (5 - 7).

1. Friedman, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:6815.
2. Chicheportiche, Y. and P. Vassalli (1994) J. Biol. Chem. 269:5512.
3. Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.
4. Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
5. Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
6. Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
7. Sasaki, T. et al. (1998) EMBO J. 17:1606.
8. Trahey, M. and Weissman, I. L. (1999) Proc. Natl. Acad. Sci. USA 96:3006.
9. Kong, W. et al. (2007) J. Cell. Physiol. 210:153.
10. Shimizu, T. et al. (2005) J. Cereb. Blood Flow Metab. 25:325.

Bioinformatics

• Uniprot:
  • NP_035280()