Renin R Antibody - BSA Free

Western Blot: Renin R Antibody [NBP1-33605] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Renin Receptor antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Renin R Antibody [NBP1-33605] - Methanol-fixed HepG2, using Renin Receptor (NBP1-33605) antibody at 1:200 dilution.

Immunocytochemistry/Immunofluorescence: Renin R Antibody [NBP1-33605] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Renin Receptor stained by Renin Receptor antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: Renin R Antibody [NBP1-33605] - Renin Receptor antibody [N3C3] detects Renin Receptor protein by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Renin Receptor stained by Renin Receptor antibody [N3C3] diluted at 1:500.Red: Tau, a Axon marker, stained by Tau antibody [287] diluted at 1:500.Blue: Fluoroshield with DAPI.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Renin R Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Renin R. The exact sequence is proprietary.
• Localization: Membrane, Single-pass type I membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP6AP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Renin R Antibody - BSA Free

• APT6M8-9
• ATP6AP2
• ATP6IP2
• ATP6M8-9
• ATP6M8-9MRXE
• ATPase H(+)-transporting lysosomal accessory protein 2
• ATPase H(+)-transporting lysosomal-interacting protein 2
• ATPase, H+ transporting, lysosomal accessory protein 2
• ATPase, H+ transporting, lysosomal interacting protein 2
• CAPER
• ELDF10
• Embryonic liver differentiation factor 10
• ER-localized type I transmembrane adaptor
• H+ transporting, lysosomal (vacuolar proton pump) membrane sectorassociated protein M8-9
• HT028
• M8-9
• MGC99577
• MRXE
• MSTP009
• N14F
• Renin R
• renin receptor
• ReninR
• vacuolar proton ATP synthase membrane sector associated protein M8-9
• V-ATPase M8.9 subunit
• XMRE

Background

This gene encodes a protein which is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Renin R Antibody (NBP1-33605)(1)

Publication using NBP1-33605

• Shiyue Pan, Lucas A C Souza, Caleb J Worker, Miriam E Reyes Mendez, Ariana Julia B Gayban, Silvana G Cooper, Alfredo Sanchez Solano, Richard N Bergman, Darko Stefanovski, Gregory J Morton, Michael W Schwartz, Yumei Feng Earley (Pro)renin receptor signaling in hypothalamic tyrosine hydroxylase neurons is required for obesity-associated glucose metabolic impairment. JCI insight 2024-03-25 [PMID: 38349753]

Bioinformatics

• Gene Symbol: ATP6AP2
• Entrez:
  • 10159(Human)
• Uniprot:
  • O75787()