Retinol Binding Protein RBP Antibody - BSA Free

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-20132] - Elevation of retinal tissue induction of visual cycle enzymes stimulated by retinoic acid 6 (STRA6, C) in chrysin-treated mice. The db/db mice were orally supplemented with 10 mg/kg of chrysin daily for 10 weeks. The db/m mice were introduced as control animals. Mouse retinal tissue extracts were subject to Western blot analysis with a primary antibody against each target protein of LRAT, RDH5, cellular retinol binding protein (CRBP), CRALBP, IRBP, or STRA6. beta-actin protein was used as an internal control. Bar graphs (mean +- SEM, n = nine independent experiments) in the bottom or right panels represent densitometric results of upper blot bands. * Values in bar graphs indicate a significant difference at pSample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Retinol Binding Protein RBP stained by Retinol Binding Protein RBP antibody (NBP2-20132) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10 um.

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-20132] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Retinol Binding Protein RBP antibody (NBP2-20132) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-20132] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Retinol Binding Protein RBP antibody (NBP2-20132) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Retinol Binding Protein RBP Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Retinol Binding Protein RBP. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Retinol Binding Protein RBP Antibody - BSA Free

• C
• Cellular retinol-binding protein
• CRABP-I
• CRBP1CRBP-I
• CRBPCellular retinol-binding protein I
• CRBPI
• RBPC
• retinol binding protein 1, cellular
• retinol-binding protein 1
• retinol-binding protein 1, cellular

Background

This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Retinol Binding Protein RBP Antibody (NBP2-20132)(1)

Publication using NBP2-20132

• Kang MK, Lee EJ, Kim YH et al. Chrysin Ameliorates Malfunction of Retinoid Visual Cycle through Blocking Activation of AGE-RAGE-ER Stress in Glucose-Stimulated Retinal Pigment Epithelial Cells and Diabetic Eyes. Nutrients 2018-08-08 [PMID: 30096827] (WB, Mouse)

Bioinformatics

• Gene Symbol: RBP1
• Uniprot:
  • P09455(Human)