RFP Antibody - BSA Free

Western Blot: RFP Antibody [NBP1-97371] - Antibody was added at 1.0ug/mL at RT for 2 hours. IRDye800(R) anti-Chicken was added at 1:20,000 at RT for 45 minutes.

Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]

Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]

Western Blot Results of RFP Antibody. Lane 1: Opal PreStained Molecular Weight Marker

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free

RFP Antibody - BSA Free Summary

• Description: Chicken products cannot be exported to Canada.
  
  
  
  RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. (Uniprot: Q9U6Y8)
• Specificity: RFP Antibody was tested by western blot.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:3000
• Application Notes: This product is designed to detect recombinant RFP. This product antibody has been tested by ELISA, SDS-PAGE, and western blot to detect RFP. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
  
  Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 30159312).
  
  Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30158698). Use in IHC-P reported in scientific literature (PMID:34389674).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30159312).

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Notes

Chicken products cannot be exported to Canada.

Alternate Names for RFP Antibody - BSA Free

• DSRED
• GFP-like chromoprotein
• Red Fluorescent Protein
• RFP

Background

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RFP Antibody (NBP1-97371)(12)

Publications using NBP1-97371

• Tai Y, Engels D, Locatelli G et al. Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions bioRxiv 2023-04-04 (IHC, Mouse)
  
  Details:
  Dilutions: 1:1000
• Skinnider M, Gautier M, Teo A et al. The Tabulae Paralytica:Multimodal single-cell and spatial atlases of spinal cord injury bioRxiv 2023-06-24 (Immunohistochemistry-Frozen, Mouse)
• Xie Q, Dasari R, Namba MD et Al. Astrocytic regulation of cocaine locomotor sensitization in EcoHIV infected mice Neuropharmacology 2024-12-02 [PMID: 39631679]
• Daniel Holl, Wing Fung Hau, Anais Julien, Shervin Banitalebi, Jannis Kalkitsas, Soniya Savant, Enric Llorens-Bobadilla, Yann Herault, Guillaume Pavlovic, Mahmood Amiry-Moghaddam, David Oliveira Dias, Christian Göritz Distinct origin and region-dependent contribution of stromal fibroblasts to fibrosis following traumatic injury in mice. Nature neuroscience 2024-07-11 [PMID: 38849523]
• Salimando GJ, Tremblay S, Kimmey BA et al. Human OPRM1 and murine Oprm1 promoter driven viral constructs for genetic access to ?-opioidergic cell types Nat Commun 2023-09-13 [PMID: 37704594] (, Immunocytochemistry/ Immunofluorescence)
• Tai YH, Engels D, Locatelli G et al. Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions Nature Metabolism 2023-07-10 [PMID: 37430025] (, Immunocytochemistry/ Immunofluorescence)
• Qu R, Gupta K, Dong D et al. Decomposing a deterministic path to mesenchymal niche formation by two intersecting morphogen gradients Developmental cell 2022-04-25 [PMID: 35421372]
• Dorst MC, DIaz-Moreno M, Dias DO Et al. Astrocyte-derived neurons provide excitatory input to the adult striatal circuitry Proceedings of the National Academy of Sciences of the United States of America 2021-08-17 [PMID: 34389674] (IHC-P)
• Helseth AR, Hernandez-Martinez R, Hall VL et al. Cholinergic neurons constitutively engage the ISR for dopamine modulation and skill learning in mice Science (New York, N.Y.) 2021-04-23 [PMID: 33888613]
• Giacometti LL, Chandran K, Figueroa LA, Barker JM Astrocyte modulation of extinction impairments in ethanol-dependent female mice Neuropharmacology 2020-11-15 [PMID: 32801026] (ICC/IF)
• Kandel C, Schmidt P, Perniss A et al. ENaC in Cholinergic Brush Cells Front Cell Dev Biol 2018-08-15 [PMID: 30159312] (IHC-WhMt)
• Anderson MA, O'Shea TM, Burda JE et al. Required growth facilitators propel axon regeneration across complete spinal cord injury Nature 2018-08-31 [PMID: 30158698] (ICC/IF)

FAQs for RFP Antibody (NBP1-97371). (Showing 1 - 1 of 1 FAQs).

1. Would this antibody detect DsRed?
   • NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.