Recombinant RFP His (N-Term) Protein

SDS-PAGE: Recombinant RFP Protein [NBP1-99583] - NBP1-99583 loaded under reducing conditions and stained with coomassive blue.

Product Discontinued

Recombinant RFP His (N-Term) Protein Summary

• Description: Full length recombinant RFP (Red Fluorescence Protein) from Discosoma with His tag on N-terminus.
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab51993)
• Preparation Method: This recombinant protein is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence of the protein.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Endotoxin was removed for in vivo injection or cell culture applications. Endotoxin level equal to or less than 0.1 ng/ug. Endotoxin was removed for in vivo injection or cell culture applications, but these applications have not been tested explicitly.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • SDS-Page
  • Western Blot
• Application Notes: This protein (DSRed) is suitable as control reagent for RFP expression studies or as a labeling reagent.
• Theoretical MW: 27.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with dH2O to 1 mg/ml

Alternate Names for Recombinant RFP His (N-Term) Protein

• DSRED
• GFP-like chromoprotein
• Red Fluorescent Protein
• RFP

Background

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for RFP Recombinant Protein (NBP1-99583)(1)

Publication using NBP1-99583

• Wang AL, Mambou EA, Kao AW The progranulin cleavage product granulin 3 exerts a dominant negative effect on animal fitness Human molecular genetics 2023-10-30 [PMID: 37903062]