Rho A/B/C Antibody (SR38-00)

Western Blot: Rho A/B/C Antibody (SR38-00) [NBP2-67325] - Analysis of Rho A/B/C on different lysates using Rho A/B/C Antibody (SR38-00) at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: Jurkat

Immunocytochemistry/Immunofluorescence: Rho A/B/C Antibody (SR38-00) [NBP2-67325] - Staining Rho A/B/C in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Rho A/B/C Antibody (SR38-00) [NBP2-67325] - Analysis of paraffin-embedded mouse stomach tissue using Rho A/B/C Antibody (SR38-00). Counter stained with hematoxylin.

Flow Cytometry: Rho A/B/C Antibody (SR38-00) [NBP2-67325] - Analysis of HepG2 cells with Rho A/B/C Antibody (SR38-00) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Rho A/B/C Antibody (SR38-00) [NBP2-67325] - Staining Rho A/B/C in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Rho A/B/C Antibody (SR38-00) [NBP2-67325] - Staining Rho A/B/C in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Rho A/B/C Antibody (SR38-00) [NBP2-67325] - Analysis of paraffin-embedded human gastric carcinoma tissue using Rho A/B/C Antibody (SR38-00). Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Rho A/B/C Antibody (SR38-00) [NBP2-67325] - Analysis of paraffin-embedded mouse colon tissue using Rho A/B/C Antibody (SR38-00). Counter stained with hematoxylin.

Product Discontinued

Rho A/B/C Antibody (SR38-00) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Rho A/B/C aa aa 1-193 / 193. (SwissProt: P08134 Human; SwissProt: P61586 Human; SwissProt: P62745 Human; SwissProt: P62746 Mouse; SwissProt: Q62159 Mouse; SwissProt: Q9QUI0 Mouse; SwissProt: P61589 Rat; SwissProt: P62747 Rat)
• Localization: Cytoplasm, Nucleus, Cell membrane, Cleavage furrow.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RHOA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Rho A/B/C Antibody (SR38-00)

• Aplysia ras-related homolog 12
• Aplysia RAS-related homolog 6
• ARH12
• ARH6
• ARH9
• ARHA
• ARHB
• ARHC
• EDFAOB
• epididymis secretory sperm binding protein
• h6
• H9
• MST081
• MSTP081
• oncogene RHO H12
• oncogene RHO H6
• oncogene RHO H9
• ras homolog gene family, member B
• ras homolog gene family, member C
• RAS-related homolog 9
• Rho cDNA clone 6
• RHO12
• rhoC GTPase
• RHOH12
• RHOH6
• RHOH9
• rho-related GTP-binding protein RhoB
• rho-related GTP-binding protein RhoC
• small GTP binding protein RhoA
• small GTP binding protein RhoC
• transforming protein RhoA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOA