RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) [PE] Summary
| Immunogen |
Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5 |
| Modification |
p Ser5 |
| Specificity |
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. |
| Isotype |
IgG1 |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
POLR2A |
| Purity |
Protein A purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Chromatin Immunoprecipitation (ChIP)
- Chromatin Immunoprecipitation Sequencing
- CyTOF-ready
- ELISA
- Flow Cytometry
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- Immunoprecipitation
- Western Blot
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
PBS |
| Preservative |
0.05% Sodium Azide |
| Purity |
Protein A purified |
Notes
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) [PE]
Background
RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for RNA Polymerase II/POLR2A Antibody (NBP2-50274PE) (0)
Secondary Antibodies
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