RNF12 Antibody

Western Blot: RNF12 Antibody [NBP2-20175] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7. 5% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RNF12 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RNF12. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RLIM
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (86%), Rat (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RNF12 Antibody

• DKFZp686N06224
• E3 ubiquitin-protein ligase RLIM
• E3 ubiquitin-protein ligase RNF12
• EC 6.3.2
• EC 6.3.2.-
• FLJ25923
• FLJ41093
• LIM domain-interacting RING finger protein
• MGC15161
• NY-REN-43
• Renal carcinoma antigen NY-REN-43
• RING finger LIM domain-binding protein
• RING finger protein 12FLJ42887
• ring finger protein, LIM domain interacting
• ring zinc finger LIM domain binding protein
• ring zinc finger protein NY-REN-43antigen
• R-LIM
• RNF12FLJ45986

Background

The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RLIM
• Uniprot:
  • Q9NVW2(Human)