Ryanodine Receptor 2 Antibody - Azide and BSA Free

Western Blot: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] - Western blot analysis of lysates from Mouse heart, using Ryanodine Receptor 2 Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

Immunohistochemistry: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] - Immunohistochemistry analysis of paraffin-embedded Human heart tissue using Ryanodine Receptor 2 Rabbit pAb at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using Ryanodine Receptor 2 Rabbit pAb at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] - Immunofluorescence analysis of paraffin-embedded mouse heart using Ryanodine Receptor 2 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Ryanodine Receptor 2 Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 4868-4967 of human Ryanodine Receptor 2 (NP_001026.2). EHTGQESYVWKMYQERC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RYR2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Ryanodine Receptor 2 Antibody - Azide and BSA Free

• arrhythmogenic right ventricular dysplasia 2
• ARVC2
• ARVD2
• cardiac muscle ryanodine receptor-calcium release channel
• Cardiac muscle-type ryanodine receptor
• cardiac-type ryanodine receptor
• hRYR-2
• islet-type ryanodine receptor
• kidney-type ryanodine receptor
• ryanodine receptor 2 (cardiac)
• Ryanodine Receptor 2
• RyR2
• RYR-2
• type 2 ryanodine receptor
• VTSIP

Background

Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The alpha1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR alpha1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR alpha1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RYR2