Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein

SDS-Page: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Recombinant 2019-nCoV Spike RBD Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.

ELISA: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Immobilized Recombinant SARS-COV-2 Spike RBD-His at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL.

HPLC: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - The purity of SARS-COV-2 Spike RBD Protein with His tag (Cat.NBP2-90982) was greater than 95% as determined by SEC-HPLC.

Surface Plasmon Resonance: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay.

• Applications: ELISA, PAGE, HPLC, SPR
• Concentration: LYOPH

Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein Summary

• Description: A bioactive partial recombinant protein with a C-Terminal His-tag and corresponding to the amino acids sequence of (319-541) of the SARS-CoV-2 Spike S1
  
  

  Source: HEK293

  RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF (Accession #YP_009724390.1)

• Details of Functionality: 1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Spike S1 RBD at 2 ug/mL (100 uL/well) can bind Human ACE-2 with a linear range of 0.001-2.96 ng/mL. 2. Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 RBD Protein with an affinity constant of 35.3 nM as determined in a SPR assay. 3. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Spike S1 RBD at 2 ug/mL (100 uL/well) can bind Human ACE-2 with a linear range of 0.001-1.69 ng/mL.
• Source: HEK293
• Protein/Peptide Type: Recombinant Protein
• Gene: S
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 0.1 EU/ug of the protein by LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • HPLC
  • SDS-Page
  • Surface Plasmon Resonance

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Alternate Names for Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein

• SARS-CoV-2

Background

The SARS-CoV-2 Spike protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The spike protein is the largest of the structural proteins, which also include the membrane (M), envelope (E), and nucleocapsid (N) proteins (1,2). The SARS-CoV-2 spike protein is a 1273 amino acid (aa) heterotrimeric class I fusion protein with each monomer having a theoretical molecular weight of approximately 180 kDa (1). The club-shaped spike protein contains several functional regions and domains including the S1 globular head region which contains the N-terminal receptor-binding domain (RBD) and the S2 stem region that contains the C-terminal fusion domain, two heptad regions, a transmembrane domain, and a cytoplasmic tail (1,2). The viral spike protein is critical for attachment of the virus with the host cell, resulting in fusion and virus entry into the cell (1,2). More specifically, the RBD of the spike protein is responsible for binding to the cell surface receptor angiotensin converting enzyme 2 (ACE2) (1,2). This spike-ACE2 interaction results in a conformational change permitting furin cleavage between the S1 and S2 domains and then cleavage at S2' by TMPRRS2, or another protease, allowing membrane fusion (1,2).

Given the critical role of the spike protein RBD in the interaction with the ACE2 receptor and viral entry, a number of neutralizing antibodies against the RBD have been developed as potential therapeutics for treating COVID-19 (3). These antibodies bind the RBD domain on the S1 subunit inhibiting the interaction with ACE2 (3). However, more studies need to be done as neutralizing antibodies can result in antibody-dependent enhancement, in which the viral entry and replication within the host cell is increased (4). One potential way to combat antibody-dependent enhancement is the use of nanobodies (4). Furthermore, there are currently several vaccine strategies that are in clinical trials, or recently federally approved, that utilize the spike protein in different forms (e.g. full length, S1 RBD, RBD-Fc, N-terminal) for protecting against SARS-CoV-2 infection (4,5). These vaccine strategies include DNA vaccines, viral vector-based vaccines, RNA vaccines, and subunit vaccines (4,5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Ho M. (2020). Perspectives on the development of neutralizing antibodies against SARS-CoV-2. Antibody Therapeutics. https://doi.org/10.1093/abt/tbaa009

4. Samrat, S. K., Tharappel, A. M., Li, Z., & Li, H. (2020). Prospect of SARS-CoV-2 spike protein: Potential role in vaccine and therapeutic development. Virus Research. https://doi.org/10.1016/j.virusres.2020.198141

5. Sternberg, A., & Naujokat, C. (2020). Structural features of coronavirus SARS-CoV-2 spike protein: Targets for vaccination. Life Sciences. https://doi.org/10.1016/j.lfs.2020.118056

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for SARS-CoV-2 Spike S1 Recombinant Protein (NBP2-90982)(1)

Publication using NBP2-90982

• Caohuy H, Eidelman O, Chen T Et al. Common cardiac medications potently inhibit ACE2 binding to the SARS-CoV-2 Spike, and block virus penetration and infectivity in human lung cells Scientific reports 2021-11-12 [PMID: 34773067]

Bioinformatics

• Gene Symbol: S