SART3 Antibody (2G2) - BSA Free Summary
Immunogen |
Recombinant full length protein (Human). |
Localization |
Mitochondrial |
Specificity |
Mitochondrial p110 (2G2) |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
SART3 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:10-1:2000
- Western Blot 2 - 10 ug/ml
|
Application Notes |
IF: Use at an assay dependent dilution. WB: Use at a concentration of 2 - 10 ug/ml. Detects a band of approximately 110 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
Theoretical MW |
110 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Cross-reacts with Human and Monkey. Not yet tested in other species.
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.4) |
Preservative |
0.08% Sodium Azide |
Concentration |
1.0 mg/ml |
Purity |
Protein G purified |
Alternate Names for SART3 Antibody (2G2) - BSA Free
Background
p110 has an approximate isoelectric point of 6.5 and co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. Close examination of the staining pattern in HeLa and Fanconi's anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi's anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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