SCAP Antibody - BSA Free

Western Blot:SCAP Antibody [NBP2-04113] -Whole cell lysate (10 ug) from HEK293T, Hep-G2, HeLa,Jurkat, and K-562 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-SCAP antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation:SCAP Antibody [NBP2-04113] -Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-SCAP antibody usedfor IP at 6 µg per reaction. SCAP was alsoimmunoprecipitated by a previous lot of this antibodyFor blotting immunoprecipitated SCAP,was used at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 30 seconds

• Reactivity: Hu
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SCAP Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human Sterol Regulatory Element Binding Protein Ceavage-Activating Protein using the numbering given in entry NP_036367.2 (GeneID 22937).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Simple Western 1:25
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SCAP Antibody - BSA Free

• KIAA0199
• PSEC0227
• SREBF chaperone
• SREBP cleavage-activating protein
• sterol regulatory element binding protein cleavage-activating protein

Background

Sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) is a molecular chaperone that plays a role in the biosynthesis of cholesterol and triglycerides (1). SCAP is a membrane protein that helps shuttle SREBP from the endoplasmic reticulum (ER) via coat protein II (COPII) vesicles to the golgi where SREBP is cleaved via protease S1P and S2P, becomes activated, and is translocated to the nucleus for transcription (1-3). The SCAP protein has a theoretical molecular weight of 140 kDa and is comprised of 1276 amino acids with an N-terminal domain containing 8 transmembrane alpha helices, where transmembrane 2-6 consist of a sterol-sensing domain, and a C-terminal domain with WD40 motif repeats (1,3). The SCAP-SREBP association plays a pivotal role in regulating lipid metabolism and lipogenesis (1,4). SREBP activation via SCAP is associated with accumulation of triglycerides and cholesterol leading to metabolic disorders including diabetes, atherosclerosis, and nonalcoholic fatty liver disease (NAFLD) (1,4). Animal models have revealed that inhibition or blocking of SCAP using antagonists may be a potential therapeutic target for hyperlipidemia and hypertriglyceridemia (1,4).

References

1. Lee, S. H., Lee, J. H., & Im, S. S. (2020). The cellular function of SCAP in metabolic signaling. Experimental & molecular medicine, 52(5), 724-729. https://doi.org/10.1038/s12276-020-0430-0

2. Cheng, X., Li, J., & Guo, D. (2018). SCAP/SREBPs are Central Players in Lipid Metabolism and Novel Metabolic Targets in Cancer Therapy. Current topics in medicinal chemistry, 18(6), 484-493. https://doi.org/10.2174/1568026618666180523104541

3. Brown, M. S., Radhakrishnan, A., & Goldstein, J. L. (2018). Retrospective on Cholesterol Homeostasis: The Central Role of Scap. Annual review of biochemistry, 87, 783-807. https://doi.org/10.1146/annurev-biochem-062917-011852

4. Moon Y. A. (2017). The SCAP/SREBP Pathway: A Mediator of Hepatic Steatosis. Endocrinology and metabolism (Seoul, Korea), 32(1), 6-10. https://doi.org/10.3803/EnM.2017.32.1.6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCAP Antibody (NBP2-04113)(4)

Publications using NBP2-04113

• Mi-Hua Liu, Xiao-Long Lin, Le-Le Xiao Excess phosphate promotes SARS‑CoV‑2 N protein‑induced NLRP3 inflammasome activation via the SCAP‑SREBP2 signaling pathway. Molecular medicine reports 2024-01-29 [PMID: 38275129]
• Li D, Liu M, Li Z et al. Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice Aging and disease 2021-06-01 [PMID: 34094640] (Human, Mouse, Immunohistochemistry)
• Muku, G E, Kusnadi, A Et al. Selective Ah receptor modulators attenuate NPC1L1-mediated cholesterol uptake through repression of SREBP-2 transcriptional activity. Lab Invest 2020-02-01 [PMID: 31417158] (WB, Human)
• Muku, G E, Blazanin, N Et al. Selective Ah receptor ligands mediate enhanced SREBP1 proteolysis to restrict lipogenesis in sebocytes. Toxicol Sci 2019-06-21 [PMID: 31225620] (IF/IHC, Mouse, Human)

Bioinformatics

• Gene Symbol: SCAP
• Entrez:
  • 22937(Human)
• Uniprot:
  • NP_036367.2(Human)