SDC3 Antibody [Unconjugated]

Syndecan‑3 was detected in immersion fixed IMR‑90 human lung fibroblast cell line (positive staining) and HL‑60 human acute promyelocytic leukemia cell line (negative staining) using Goat Anti-Human Syndecan‑3 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3539) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SDC3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Syndecan-3
  Gln48-Lys383
  Accession # O75056
• Specificity: Detects human Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Syndecan-3 is observed and less than 1% cross-reactivity with recombinant mouse Syndecan-4 and recombinant human Syndecan-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SDC3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SDC3 Antibody [Unconjugated]

• KIAA0468
• N-Syndecan
• SDC3
• SDCN
• SYND3syndecan proteoglycan 3
• syndecan 3 (N-syndecan)
• syndecan 3
• syndecan neural type
• Syndecan3
• Syndecan-3

Background

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1‑3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1‑3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of human Syndecan-3 shares 83%, 83%, 92%, 91% and 91% aa identity with of mouse, rat, equine, bovine and canine Syndecan-3, respectively. Splice isoforms of 384 aa and 346 aa, containing either a 28 aa substitution for aa 1‑86 or deletion of aa 1‑96, have been reported (4). Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120‑150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta , it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11‑13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).

1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
2. Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
3. Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
4. ENTREZ protein Accession # O75056, EAX076736 and EAX07637.
5. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
6. Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
7. Hienola, A. et al. (2006) J. Cell Biol. 174:569.
8. Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
9. Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
10. Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
11. Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
12. Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
13. Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
14. Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SDC3 Antibody (AF3539)(4)

Publications using AF3539

• LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer Cell Rep, 2020-10-13;33(2):108253. 2020-10-13 [PMID: 33053339] (CyTof, Human)
• A Hudák, E Kusz, I Domonkos, K Jósvay, AT Kodamullil, L Szilák, M Hofmann-Ap, T Letoha Contribution of syndecans to cellular uptake and fibrillation of alpha-synuclein and tau Sci Rep, 2019-11-12;9(1):16543. 2019-11-12 [PMID: 31719623] (Co-Immunoprecipitation, Human)
• Brian DellaValle, Alba Manresa-Arraut, Henrik Hasseldam, Allan Stensballe, Jørgen Rungby, Agnete Larsen et al. Detection of Glycan Shedding in the Blood: New Class of Multiple Sclerosis Biomarkers? Frontiers in Immunology 2018-06-04 [PMID: 29915593]
• H Fan, L Qiao, KD Kang, J Fan, W Wei, G Luo Attachment and Post-Attachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission J. Virol., 2017-06-09;0(0):. 2017-06-09 [PMID: 28404852] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SDC3
• Uniprot:
  • O75056()