Secernin-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Secernin-1 Antibody [NBP1-77187] - Human kidney tissue with Secernin-1 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: Secernin-1 Antibody - BSA Free [NBP1-77187] - Immunofluorescence of Secernin-1 in Human Kidney cells with Secernin-1 antibody at 20 ug/mL.

Western Blot: Secernin-1 Antibody - BSA Free [NBP1-77187] - Western blot analysis of SCRN1 in human kidney tissue lysate with SCRN1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Secernin-1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human SCRN1. The immunogen is located within amino acids 320 - 370 of SCRN1. Amino Acid Squence: CPAKKEPRFQEKPDRRHELYK
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCRN1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  Secernin-1 Peptide (NBP1-77187PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Secernin-1 Antibody - BSA Free

• KIAA0193SES1
• secernin 1
• secernin-1

Background

SCRN1 was first identified as a cytosolic protein that is involved in the regulation of exocytosis from peritoneal mast cells. More recent studies have shown that SCRN1 expression is upregulated in gastric cancer cell lines and may possess epitopes that could function as tumor-associated antigens, potentially providing targets for cancer vaccines in the treatment of gastric cancers. Another report indicates that decreased expression of SCRN1 via RNAi expression resulted in significantly lower rates of cell growth in colorectal cancer cell lines, and increased SCRN1 expression in patients with colorectal cancer correlated with poor prognosis, suggesting that SCRN1 may also be involved in the regulation of cell growth and might be useful as a prognostic tool.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCRN1
• Uniprot:
  • NP_001138985(Human)