SGK3 Antibody - BSA Free

Immunohistochemistry-Paraffin: SGK3 Antibody [NBP1-80760] - Staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SGK3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DVAEMYDNILHKPLSLRPGVSLTAWSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSY
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SGK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  SGK3 Protein (NBP1-80760PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SGK3 Antibody - BSA Free

• CISK
• DKFZp781N0293
• EC 2.7.11
• EC 2.7.11.1
• serine/threonine-protein kinase Sgk3
• serum/glucocorticoid regulated kinase 3
• serum/glucocorticoid regulated kinase family, member 3
• serum/glucocorticoid regulated kinase-like
• Serum/glucocorticoid-regulated kinase 3
• Serum/glucocorticoid-regulated kinase-like
• SGK2
• SGK3
• SGKLcytokine-independent survival kinase

Background

SGKL/SGK3, also known as CISK for cytokine-independent survival kinase, is a SGK type protein kinase. The protein contains a lipid-binding phox homology (PX) domain, which is required for targeting SGKL/SGK3 protein to the endosomal compartment. SGKL/SGK3 functions downstream of the phosphoinositide 3-kinase (PI3K) cascade and has been implicated in cell survival. The protein has been shown to be phosphorylated and activated by 3-phosphoinositide-dependent protein kinase 1 (PDK1), and to a lesser extent insulin-like growth factor-1, in vitro. Activated SGKL/SGK3 has been shown to phosphorylate glycogen synthase kinase 3 beta (GSK-3beta) in vitro. SGKL/SGK3 expression has been documented in many human tissues but was found to be most abundant in lung. ESTs have been isolated from human tissue libraries, including normal liver, breast and thyroid and cancerous spleen, ear and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SGK3 Antibody (NBP1-80760)(2)

Publications using NBP1-80760

• Nishad R, Mukhi D, Kethavath S et al. Podocyte derived TNF-alpha mediates monocyte differentiation and contributes to glomerular injury FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-12-01 [PMID: 36421039]
• Li X, Su Q, Pan Y Overcharged lipid metabolism in mechanisms of antitumor by Tremella fuciformis‑derived polysaccharide International journal of oncology 2023-01-01 [PMID: 36416344] (WB, Mouse)
  
  Details:
  Dilutions: 1:1000

Bioinformatics

• Gene Symbol: SGK3
• Entrez:
  • 100533105(Human)
  • 23678(Human)