SIAH1 Antibody - BSA Free

Western Blot: SIAH1 Antibody [NBP2-20356] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SIAH1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Rt, Bv, Pm, RM, Xp, Ze
• Applications: WB, Func, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SIAH1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SIAH1. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Rat (99%), Xenopus (99%), Bovine (100%), Chimpanzee (100%), Zebrafish (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIAH1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Reported in scientific literature (PMID: 25587023)
  • Proximity Ligation Assay Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SIAH1 Antibody - BSA Free

• E3 ubiquitin-protein ligase SIAH1
• EC 6.3.2
• EC 6.3.2.-
• FLJ08065
• hSIAH1
• HUMSIAH
• seven in absentia homolog 1 (Drosophila)
• Seven in absentia homolog 1
• Siah-1
• SIAH1A
• siah-1a

Background

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SIAH1 Antibody (NBP2-20356)(1)

Publication using NBP2-20356

• Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014-01-01 [PMID: 25587023] (IP, WB, Human)

Bioinformatics

• Gene Symbol: SIAH1
• Uniprot:
  • Q8IUQ4(Human)