SLC20A1 Antibody - BSA Free

Western Blot: SLC20A1 Antibody [NBP1-32252] - Unboiled U87-MG cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC20A1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Western Blot: SLC20A1 Antibody [NBP1-32252] - Unboiled U87-MG cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC20A1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Immunohistochemistry-Paraffin: SLC20A1 Antibody [NBP1-32252] - Paraffin-embedded mouse mammary gland. SLC20A1 antibody [N3C2], Internal diluted at 1:500.

Immunohistochemistry-Paraffin: SLC20A1 Antibody [NBP1-32252] - Paraffin-embedded AGS xenograft. SLC20A1 antibody [N3C2], Internal dilution: 1:500.

• Reactivity: Hu, Mu, Rt, Bv, Fe, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SLC20A1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SLC20A1. The exact sequence is proprietary.
• Localization: Membrane, Multi-pass membrane protein
• Predicted Species: Rat (94%), Bovine (95%), Rhesus Macaque (98%), Feline (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC20A1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (95%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SLC20A1 Antibody - BSA Free

• FLJ41426
• Gibbon ape leukemia virus receptor 1
• Glvr-1
• GLVR1DKFZp686J2397
• Leukemia virus receptor 1 homolog
• Phosphate transporter 1
• PiT-1PIT1
• sodium-dependent phosphate transporter 1
• solute carrier family 20 (phosphate transporter), member 1
• Solute carrier family 20 member 1

Background

Retrovirus receptors allow infection of human and murine cells by various retroviruses. The receptors that have been identified at the molecular level include CD4 (MIM 186940) for human immunodeficiency virus, Rec1 for murine ecotropic virus, and GLVR1 for gibbon ape leukemia virus (see MIM 182090). These 3 proteins show no homology to one another at the DNA or protein level. GLVR1 is a sodium-dependent phosphate symporter.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for SLC20A1 Antibody (NBP1-32252). (Showing 1 - 1 of 1 FAQ).

1. The predicted molecular weight of SLC20A1 protein is about 74 kDa. Why can this antibody (NBP1-32252) detect two bands in different cell types? The translational modification seems not to explain these variations in MW.  ..show answer..
   • SLC20A1 is a transmembrane protein and, as well as post-translational modification (1 extra phosphorylation site), another factor responsible for its differential migration in different species might be the differences in the integration of this protein in the membrane. It has 10 transmembrane regions and 11 topological regions in the sequence and the position of amino acids varies in mouse and human with respect to these regions in this protein.

Bioinformatics

• Gene Symbol: SLC20A1
• Entrez:
  • 6574(Human)
  • 20515(Mouse)
• Uniprot:
  • Q8WUM9(Human)