Slug Antibody - BSA Free

Western Blot: Slug Antibody - BSA Free [NB100-80828] - Human kidney cell lysate with Slug antibody at in (A) 1 and (B) 2 ug/ml.

Immunohistochemistry-Paraffin: Slug Antibody - BSA Free [NB100-80828] - Staining of human pancreas after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Slug Antibody - BSA Free Summary

• Immunogen: A 14 amino acid synthetic peptide near the center of human Slug. The immunogen is located within amino acids 70 - 120 of Slug.
• Epitope: Near the center, aa 70-120
• Specificity: 14 amino acid peptide from near the center of human Slug
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNAI2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Use in ICC/IF reported in scientific literature (PMID 27524413).
• Control:

  Slug Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Slug Antibody - BSA Free

• MGC10182
• Neural crest transcription factor Slug
• Protein snail homolog 2
• slug homolog, zinc finger protein (chicken)
• Slug
• SLUGH
• SLUGH1
• SLUGzinc finger protein
• SNAI2
• snail 2
• snail homolog 2 (Drosophila)
• SNAIL2
• WS2D
• zinc finger protein SNAI2

Background

The zinc finger transcription factor 'snail' was first identified in Drosophila and, along with 'twist', a basic helix-loop-helix transcription factor, is indispensable for mesoderm formation. Snail is a repressor of mouse E-cadherin transcription, with expression of Snail inversely correlated with expression of E-cadherin. Abnormal expression of SNAIL could underlie the tumorigenic conversion of epithelia associated with loss of E-cadherin expression through screening mouse and human cell lines and by in situ hybridization of primary human tumors undergoing malignant progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Slug Antibody (NB100-80828)(1)

Publication using NB100-80828

• Hwang MH, Cho KH, Jeong KJ et al. RCP induces Slug expression and cancer cell invasion by stabilizing beta1 integrin. Oncogene. 2016-08-15 [PMID: 27524413] (IF/IHC, ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: SNAI2
• Entrez:
  • 6591(Human)
  • 20583(Mouse)
• Uniprot:
  • O43623(Human)