Slug Antibody - BSA Free

Western Blot: Slug Antibody [NBP2-27182] - Analysis of extracts from HepG2 cells using Slug antibody (NBP2-27182) at 1:1000.

Immunohistochemistry: Slug Antibody [NBP2-27182] - Analysis of liver cancer using Slug antibody (NBP2-27182) at 1:25. Image objective 400X.

Immunohistochemistry: Slug Antibody [NBP2-27182] - Analysis of gastric cancer using Slug antibody (NBP2-27182) at 1:25. Image objective 100X.

Immunohistochemistry: Slug Antibody [NBP2-27182] - Analysis of gastric cancer using Slug antibody (NBP2-27182) at 1:25. Image objective 400X.

• Reactivity: Hu, Mu, Ca, Eq
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Slug Antibody - BSA Free Summary

• Immunogen: Amino acids 82-110 of human SNAIL2 (SNAI2) were used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNAI2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:2000
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat SNAIL2 (SNAI2) (89%). Mouse reactivity reported in scientific literature (PMID: 26240017)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Slug Antibody - BSA Free

• MGC10182
• Neural crest transcription factor Slug
• Protein snail homolog 2
• slug homolog, zinc finger protein (chicken)
• Slug
• SLUGH
• SLUGH1
• SLUGzinc finger protein
• SNAI2
• snail 2
• snail homolog 2 (Drosophila)
• SNAIL2
• WS2D
• zinc finger protein SNAI2

Background

Zinc finger transcription factor SNAIL2 (SNAI2, Slug) is found to have some functional significance in formation of axis and mesoderm in Drosophila. SNAIL2 and Twist act as negative regulators of level of Cerberus RNA, which encodes a Nodal, bone morphogenic protein (BMP) and WNT inhibitor. mRNA levels of SNAIL2 are regulated by NF-kappaB in early embryo. Partial functional redundancy between Twist, SNAIL2 and SNAIL1 may be developmentally and physiologically significant given their roles in the induction and progression of various forms of epithelial-mesenchymal transition and the metastasis of cancer cells, the regulation of apoptosis, and their ability to generate stem cell like behaviors in immortalized human epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Slug Antibody (NBP2-27182)(4)

Publications using NBP2-27182

• Laura Lecce, Yang Xu, Bhargavi V’Gangula, Nirupama Chandel, Venu Pothula, Axelle Caudrillier, Maria Paola Santini, Valentina d’Escamard, Delaine K. Ceholski, Przemek A. Gorski, Lijiang Ma, Simon Koplev, Martin Mæng Bjørklund, Johan L.M. Björkegren, Manfred Boehm, Jacob Fog Bentzon, Valentin Fuster, Ha Won Kim, Neal L. Weintraub, Andrew H. Baker, Emily Bernstein, Jason C. Kovacic Histone deacetylase 9 promotes endothelial-mesenchymal transition and an unfavorable atherosclerotic plaque phenotype The Journal of Clinical Investigation 2021-08-02 [PMID: 34338228]
• Itinteang T, Tan CES, van Schaijik B et al. Proliferating Infantile Hemangioma Tissues and Primary Cell Lines Express Markers Associated with Endothelial-to-Mesenchymal Transition Plast Reconstr Surg Glob Open 2020-02-01 [PMID: 32309069] (IHC-P, Human)
• Nakayama M, Sakai E, Echizen K et al. Intestinal cancer progression by mutant p53 through the acquisition of invasiveness associated with complex glandular formation. Oncogene 2017-01-01 [PMID: 28628120] (IF/IHC, Mouse)
• Ke CY, Xiao WL, Chen CM et al. IRF6 is the mediator of TGFbeta3 during regulation of the epithelial mesenchymal transition and palatal fusion. Sci Rep. 2015-08-05 [PMID: 26240017] (IHC-P, Mouse)
  
  Details:
  SLUG/SNAI2 antibody used for immunohistochemical staining of palatal shelves from E13.5 mouse embryos that were infected with shLuc, shIrf6 lentivirus, or treated with 20 ng/ml TGFbeta3 for 24 hours (Figure 2a).

Bioinformatics

• Gene Symbol: SNAI2