Smad2/3 [p Ser465, p Ser467] Antibody (1074A) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 30 minutes. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Smad2/3 (S465/S467) Monoclonal Antibody (Catalog # MAB8935) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Smad2/3 (S465/S467) at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clone: 1074A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Smad2/3 [p Ser465, p Ser467] Antibody (1074A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human Smad2 S465/S467 site
  Accession # NP_005892
• Modification: p Ser465, p Ser467
• Specificity: Detects human Smad2 and Smad3 when dually phosphorylated at S465 and S467 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad2/3 [p Ser465, p Ser467] Antibody (1074A) [Unconjugated]

• hMAD-2
• hSMAD2
• JV18
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2MGC34440
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smad2/3

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-beta or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad2/3 Antibody (MAB8935)(12)

Publications using MAB8935

• Yoshida M, Kokubun T, Sato K et al. DPP-4 Inhibitors Attenuate Fibrosis After Glaucoma Filtering Surgery by Suppressing the TGF-?/Smad Signaling Pathway Investigative ophthalmology & visual science 7/3/2023 [PMID: 37405760]
• Passanha FR, Geuens T, LaPointe VLS Cadherin-11 Influences Differentiation in Human Mesenchymal Stem Cells by Regulating the Extracellular Matrix Via the TGF?1 Pathway Stem cells (Dayton, Ohio) 7/27/2022 [PMID: 35416252]
• F Lombardi, FR Augello, P Palumbo, E Mollsi, M Giuliani, AM Cimini, MG Cifone, B Cinque Soluble Fraction from Lysate of a High Concentration Multi-Strain Probiotic Formulation Inhibits TGF-&beta1-Induced Intestinal Fibrosis on CCD-18Co Cells Nutrients, 2021-03-09;13(3):. 2021-03-09 [PMID: 33803197] (Western Blot, Human)
• Neha Ahuja, Cheemala Ashok, Subhashis Natua, Deepak Pant, Anna Cherian, Madhura R Pandkar et al. Hypoxia-induced TGF-beta –RBFOX2–ESRP1 axis regulates human MENA alternative splicing and promotes EMT in breast cancer NAR Cancer 10/22/2020 [PMID: 33089214]
• Neha Ahuja, Cheemala Ashok, Subhashis Natua, Deepak Pant, Anna Cherian, Madhura R Pandkar et al. Hypoxia-induced TGF-beta –RBFOX2–ESRP1 axis regulates human MENA alternative splicing and promotes EMT in breast cancer NAR Cancer 2020-10-22 [PMID: 33089214] (ChIP (chromatin immunoprecipit, Human)
• LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles Nat Commun, 2020-05-11;11(1):2331. 2020-05-11 [PMID: 32393780] (Western Blot, Human)
• P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule Cell Rep, 2019-12-10;29(11):3488-3505.e9. 2019-12-10 [PMID: 31825831] (ICC, Western Blot, Human)
• S Zhou, SD da Silva, PM Siegel, A Philip CD109 acts as a gatekeeper of the epithelial trait by suppressing epithelial to mesenchymal transition in squamous cell carcinoma cells in vitro Sci Rep, 2019-11-06;9(1):16317. 2019-11-06 [PMID: 31695056] (Western Blot, Human)
• J Tan, Y Wang, S Wang, S Wu, Z Yuan, X Zhu Label-free quantitative proteomics identifies transforming growth factorβ1 (TGF-?1) as an inhibitor of adipogenic transformation in OP9-DL1 cells and primary thymic stromal cells Cell Biosci, 2019-06-14;9(0):48. 2019-06-14 [PMID: 31249661] (ChIP, Mouse)
• K Nakai, S Karita, J Igarashi, I Tsukamoto, K Hirano, Y Kubota COA-Cl prevented TGF-?1-induced CTGF expression by Akt dephosphorylation in normal human dermal fibroblasts, and it attenuated skin fibrosis in mice models of systemic sclerosis J. Dermatol. Sci., 2019-03-12;0(0):. 2019-03-12 [PMID: 30954335] (Western Blot, Mouse)
• Yinmin Wang, Xiuxia Wang, Xianyu Zhou, Zhu Zhu, Jun Yang, Fei Liu Suppressive effect mediated by human adipose-derived stem cells on T cells involves the activation of JNK International Journal of Molecular Medicine 10/24/2018 [PMID: 30365063]
• Tao Cheng Neuronal Protein 3.1 Deficiency Leads to Reduced Cutaneous Scar Collagen Deposition and Tensile Strength due to Impaired Transforming Growth Factor-?1 to -?3 Translation Am. J. Pathol, 2016-12-08;0(0):. 2016-12-08 [PMID: 27939132] (Western Blot, Mouse)

Review for Smad2/3 Antibody (MAB8935) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pauline Jeannot WB Human 08/21/2024

Summary

• Application: Western Blot
• Sample Tested: primary astrocytes
• Species: Human
• Lot: CJRA0323041

Comments

• Comments: Lysates from primary human astrocytes

Bioinformatics

• Uniprot:
  • NP_005892()